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First isolation of bovine coronavirus with a three-amino-acid deletion in the N gene causing severe respiratory and digestive disease in calve

文献类型： 外文期刊

作者： Li, Siyuan 1 ; Yuan, Xuesong 1 ; Mao, Li 1 ; Cai, Xuhang 1 ; Xu, Xingang 2 ; Li, Jizong 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Jiangsu Key Lab Food Qual & Safety,State Key Lab C, Nanjing, Peoples R China

2.Northwest A&F Univ, Coll Vet Med, Yangling, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, Nanjing, Peoples R China

4.Jiangsu Univ, Inst Life Sci, Sch Food & Biol Engn, Zhenjiang, Peoples R China

5.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

关键词： bovine coronavirus; pathogenicity; N gene; mutation; GIIb genotype

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： Bovine coronavirus (BCoV), a persistent threat to global cattle industry, has caused significant economic losses worldwide. In this study, a viral strain was isolated from the intestinal content of a diseased calve, and identified by cytopathic effects observation, indirect immunofluorescence assay and electron microscopy. Results showed that BCoV NXWZ2310 belonging to the GIIb genotype and has a three-amino-acid deletion in the serine-rich region of the N gene. Importantly, the BCoV NXWZ2310 strain exhibited strong pathogenicity, causing nasal discharge and watery diarrhea in calves for 8 and 10 days, respectively. Viral shedding was detected in nasal, throat and rectal swabs at levels reaching 106.228 copies/mL, 105.0 copies /mL and 106.692 copies/mL, respectively. Pathological examination showed that NXWZ2310 resulted in parenchymal lesions of the pulmonary lobe and significant intestinal lesions. Both the lungs and intestines displayed marked microscopic lesions with clear viral antigens present. BCoV NXWZ2310 strain with N-gene deletion mutations, caused severe respiratory and digestive disease in calves. Therefore, effective strategies are needed for the prevention and control of this isolate.

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