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Spermatogonia From Cryopreserved Testes of Critically Endangered Chinese Sturgeon Efficiently Colonized and Preferentially Proliferated in the Recipient Gonads of Yangtze Sturgeon

文献类型： 外文期刊

作者： Ye, Huan 1 ; Takeuchi, Yutaka 2 ; Du, Hao 1 ; Yue, Huamei 1 ; Ruan, Rui 1 ; Li, Chuangju 1 ; Wei, Qiwei 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs, Wuhan 430223, Peoples R China

2.Kanazawa Univ, Fac Biol Sci & Technol, Noto Ctr Fisheries Sci & Technol, Kanazawa, Ishikawa 9270552, Japan

关键词： Xenogenic transplantation; Spermatogonia; Chinese sturgeon; Cryopreservation

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.727； 五年影响因子：3.782 ）

ISSN： 1436-2228

年卷期： 2022 年 24 卷 1 期

页码：

收录情况： SCI

摘要： The critically endangered Chinese sturgeon, Acipenser sinensis, presents late sexual maturity and has a large body size. Germ cell transplantation is a powerful technique for the production of gametes from large-bodied species in closely related recipients with a smaller body size and shorter generation time. To accelerate reproduction of Chinese sturgeon, donor spermatogonia collected from the cryopreserved testes of 3-year-old Chinese sturgeon were intraperitoneally transplanted into 7-8 days post-hatch larvae of Yangtze sturgeon (Acipenser dabryanus) with shorter generation interval. At 2 months post-transplantation (mpt), donor spermatogonia had colonized in the 81.25% of recipient gonads, with average numbers about two times those of endogenous primordial germ cells. Within the next 2 months, the rate of endogenous germ cell division in females (2-3 times) was faster than that in males (once), whereas colonized donor-derived spermatogonia divided about 2-3 times and twice in recipient females and males, respectively. Furthermore, the expression of germ cell-related genes, dazl, dead end, and vasa, in transplanted fish was higher than that in non-transplanted fish, suggesting the incorporation and proliferation donor spermatogonia in recipient. At 18 mpt, donor-derived spermatogonia survived in the 75.00% of recipient gonads. These results showed that the somatic microenvironment of Yangtze sturgeon gonad can support the long-term colonization, proliferation, and survival of xenogeneic germ cells. Thus, this study suggested that small-bodied Yangtze sturgeon is promising recipient as surrogate for Chinese sturgeon gamete production.

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