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Effects of sperm concentration on the quality of cryopreserved sperm in Yangtze sturgeon (Acipenser dabryanus)

文献类型： 外文期刊

作者： Zhu, Shuang 1 ; Ye, Huan 2 ; Li, Xin 2 ; Qiao, Xinmei 2 ; Xiong, Wei 2 ; Zhong, Jia 2 ; Shen, Li 2 ; Du, Hao 2 ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs China, Wuhan 430223, Peoples R China

关键词： Yangtze sturgeon; Sperm cryopreservation; Sperm concentration; Sperm quality; Flow cytometry

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 2352-5134

年卷期： 2024 年 36 卷

页码：

收录情况： SCI

摘要： The Yangtze sturgeon ( Acipenser dabryanus ) is extinct in the wild; however, artificial propagation in captivity under fully controlled conditions has been successful. Sperm cryopreservation is an effective method for germplasm conservation and aquaculture. Sperm concentration affects the quality of cryopreserved spermatozoa. Thus, in this study, we optimized the concentration (0.25 x 10 9 , 0.5 x 10 9 , 1.0 x 10 9 , and 2.0 x 10 9 spermatozoa [spz]/mL) for Yangtze sturgeon sperm cryopreservation in a 0.5 mL plastic straw. The greatest value for the kinetic parameters was achieved when sperm were cryopreserved at 0.5 x 10 9 spz/mL. In comparison to other concentrations, there was a notable increase in both acrosomal and plasma membrane integrity when sperm was cryopreserved at a concentration of 0.5 x 10 9 spz/mL. No significant difference of superoxide dismutase and glutathione peroxidase enzyme activity was present in post-thaw sperm, except in sperm cryopreserved at a concentration of 2.0 x 10 9 spz/mL. Catalase activity was significantly higher in post-thaw sperm at a concentration of 0.5 x 10 9 spz/mL than at other concentrations. Excluding sperm cryopreserved at 2.0 x 10 9 spz/mL, the fertilization rate of cryopreserved sperm was not significantly different among the different concentrations. This concentration optimization improves cryopreserved sperm quality and standardizes sperm cryopreservation procedures for the Yangtze sturgeon. These findings are especially beneficial for the establishment of a sperm cryobank.

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