Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant
文献类型： 外文期刊
作者： Latif, Muhammad Faizan 1 ; Tan, Jingquan 1 ; Zhang, Wang 1 ; Yang, Wenxuan 1 ; Zhuang, Tinghui 1 ; Lu, Wenlong 1 ; Qiu, Yanglin 1 ; Du, Xinying 1 ; Zhuang, Xinjian 1 ; Zhou, Tong 1 ; Kundu, Jiban K. 3 ; Yin, Jinlong 1 ; Xu, Kai 1 ;
作者机构： 1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Pathogens & Ecosyst, Nanjing 210023, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China
3.Crop Res Inst, Plant Virus & Vector Interact, Ctr Plant Virus Res, Drnovska 507, Prague 16106, Czech Republic
4.Czech Acad Sci, Ctr Plant Virus Res, Lab Virol, Inst Expt Bot, Rozvojova 263, Prague 16502, Czech Republic
关键词： MicroRNA; Potyvirus; SMV; P1 gene; Transgenic; Virus resistance
期刊名称：TRANSGENIC RESEARCH （ 影响因子：3.0； 五年影响因子：2.7 ）
ISSN： 0962-8819
年卷期： 2024 年
页码：
收录情况： SCI
摘要： RNA silencing is an innate immune mechanism of plants against invasion by viral pathogens. Artificial microRNA (amiRNA) can be engineered to specifically induce RNA silencing against viruses in transgenic plants and has great potential for disease control. Here, we describe the development and application of amiRNA-based technology to induce resistance to soybean mosaic virus (SMV), a plant virus with a positive-sense single-stranded RNA genome. We have shown that the amiRNA targeting the SMV P1 coding region has the highest antiviral activity than those targeting other SMV genes in a transient amiRNA expression assay. We transformed the gene encoding the P1-targeting amiRNA and obtained stable transgenic Nicotiana benthamiana lines (amiR-P1-3-1-2-1 and amiR-P1-4-1-2-1). Our results have demonstrated the efficient suppression of SMV infection in the P1-targeting amiRNA transgenic plants in an expression level-dependent manner. In particular, the amiR-P1-3-1-2-1 transgenic plant showed high expression of amiR-P1 and low SMV accumulation after being challenged with SMV. Thus, a transgenic approach utilizing the amiRNA technology appears to be effective in generating resistance to SMV.
- 相关文献
作者其他论文 更多>>
-
Regional maize suitability based on soil water and salt content inversion by integrating machine and transfer learnings in Xinjiang
作者：Wang, Shibin;Li, Yi;Li, Tanyi;Lu, Wenlong;Qi, Xingyun;Li, Yi;Xie, Xiangwen;Sa, Renna;Guo, Tongkai;Pulatov, Alim;Javlonbek, Ishchanov;Tang, Darrell W. S.;Siddique, Kadambot H. M.
关键词：Maize; Soil water and salt contents inversion; Water-salinity suitability index; Machine learning; Transfer learning
-
Loss-of-Function of Two PD-Associated Proteins Confers Resistance to Rice Stripe Virus
作者：Lu, Hong;Ge, Panpan;Ma, Yonghuan;Cui, Feng;Ge, Panpan;Ma, Yonghuan;Cui, Feng;Lin, Feng;Zhou, Tong;Jing, Dedao
关键词：flotillin; importin alpha; PD-associated protein; plasmodesmata; rice; rice stripe virus
-
Protease activity of NIa-Pro determines systemic pathogenicity of clover yellow vein virus
作者：Zhuang, Xinjian;Zhang, Wenyi;Xu, Shuqi;Yang, Wenxuan;Yin, Jinlong;Zhou, Tong;Xu, Kai;Yang, Wenxuan;Zhou, Tong;Kundu, Jiban K.
关键词：CIYVV; Infectious clone; NIa-pro; PVX; Nicotiana benthamiana
-
Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector
作者：Du, Linlin;Li, Tao;Wang, Yunyue;Du, Linlin;Li, Tao;Li, Chenyang;Lan, Ying;Lin, Feng;Zhou, Yijun;Zhou, Tong;Guo, Wei;Xu, Kai;Kundu, Jiban Kumar;Du, Linlin;Zhou, Tong
关键词：Mixed sample test; RSV; SRBSDV; Sentinel surveillance; SBPH
-
RNA interference-based dsRNA application confers prolonged protection against rice blast and viral diseases, offering a scalable solution for enhanced crop disease management
作者：Chen, Pan;Ding, Shaochen;Du, Ruonan;Hu, Xiaoxiao;Zhang, Han;Yu, Hanxi;Xu, Le;Niu, Dongdong;Liu, Hongxia;Zhao, Hongwei;Lan, Ying;Li, Chenyang;Lin, Feng;Du, Linlin;Zhou, Tong;Umida, Isashova;Ray, Rumiana;Liu, Tong;Liang, You
关键词：gene silence;
MoPth11 ; plant protection; rice blast; rice stripe disease; RNAi; RSV; SIGS; small RNA -
Establishment of chromatin architecture interplays with embryo hypertranscription
作者：Yu, Guang;Xu, Kai;Xia, Weikun;Zhang, Ke;Xu, Qianhua;Li, Lijia;Liu, Ling;Liu, Bofeng;Du, Zhenhai;Fan, Qiang;Lai, Fangnong;Wang, Wenying;Wang, Lijuan;Kong, Feng;Xie, Wei;Yu, Guang;Xu, Kai;Xia, Weikun;Zhang, Ke;Xu, Qianhua;Li, Lijia;Liu, Ling;Liu, Bofeng;Du, Zhenhai;Fan, Qiang;Lai, Fangnong;Wang, Wenying;Wang, Lijuan;Kong, Feng;Xie, Wei;Lin, Zili;Chen, Xia;Wang, Chao;Dai, Haiqiang;Wang, Huili
关键词：
-
Perception of viral infections and initiation of antiviral defence in rice
作者：Huang, Yu;Sun, Xi;Li, Jiahao;Yao, Shengze;Wei, Chunhong;Li, Yi;Yang, Jialin;Yang, Jialin;Sun, Xi;Li, Jiahao;Han, Yanhong;Chen, Changtian;Li, Yi;Cao, Xiaoqiang;Xie, Qi;Cao, Xiaoqiang;Xie, Qi;Du, Linlin;Li, Shuo;Ji, Yinghua;Zhou, Tong;Wang, He;Wang, Wenming;Han, Jia-jia;Xie, Qi;Yang, Zhirui;Huang, Yu
关键词：
