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Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant

文献类型： 外文期刊

作者： Latif, Muhammad Faizan 1 ; Tan, Jingquan 1 ; Zhang, Wang 1 ; Yang, Wenxuan 1 ; Zhuang, Tinghui 1 ; Lu, Wenlong 1 ; Qiu, Yanglin 1 ; Du, Xinying 1 ; Zhuang, Xinjian 1 ; Zhou, Tong 1 ; Kundu, Jiban K. 3 ; Yin, Jinlong 1 ; Xu, Kai 1 ;

作者机构： 1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Pathogens & Ecosyst, Nanjing 210023, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China

3.Crop Res Inst, Plant Virus & Vector Interact, Ctr Plant Virus Res, Drnovska 507, Prague 16106, Czech Republic

4.Czech Acad Sci, Ctr Plant Virus Res, Lab Virol, Inst Expt Bot, Rozvojova 263, Prague 16502, Czech Republic

关键词： MicroRNA; Potyvirus; SMV; P1 gene; Transgenic; Virus resistance

期刊名称：TRANSGENIC RESEARCH （ 影响因子：3.0； 五年影响因子：2.7 ）

ISSN： 0962-8819

年卷期： 2024 年

页码：

收录情况： SCI

摘要： RNA silencing is an innate immune mechanism of plants against invasion by viral pathogens. Artificial microRNA (amiRNA) can be engineered to specifically induce RNA silencing against viruses in transgenic plants and has great potential for disease control. Here, we describe the development and application of amiRNA-based technology to induce resistance to soybean mosaic virus (SMV), a plant virus with a positive-sense single-stranded RNA genome. We have shown that the amiRNA targeting the SMV P1 coding region has the highest antiviral activity than those targeting other SMV genes in a transient amiRNA expression assay. We transformed the gene encoding the P1-targeting amiRNA and obtained stable transgenic Nicotiana benthamiana lines (amiR-P1-3-1-2-1 and amiR-P1-4-1-2-1). Our results have demonstrated the efficient suppression of SMV infection in the P1-targeting amiRNA transgenic plants in an expression level-dependent manner. In particular, the amiR-P1-3-1-2-1 transgenic plant showed high expression of amiR-P1 and low SMV accumulation after being challenged with SMV. Thus, a transgenic approach utilizing the amiRNA technology appears to be effective in generating resistance to SMV.

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