文献类型： 外文期刊

作者： Yu, Guang ¹ ; Xu, Kai ¹ ; Xia, Weikun ¹ ; Zhang, Ke ¹ ; Xu, Qianhua ¹ ; Li, Lijia ¹ ; Lin, Zili ³ ; Liu, Ling ¹ ; Liu, Bofeng ¹ ; Du, Zhenhai ¹ ; Chen, Xia ⁴ ; Fan, Qiang ¹ ; Lai, Fangnong ¹ ; Wang, Wenying ¹ ; Wang, Lijuan ¹ ; Kong, Feng ¹ ; Wang, Chao ⁵ ; Dai, Haiqiang ⁵ ; Wang, Huili ⁶ ; Xie, Wei ¹ ;

作者机构： 1.Tsinghua Univ, Ctr Stem Cell Biol & Regenerat Med, Sch Life Sci, MOE Key Lab Bioinformat,New Cornerstone Sci Lab, Beijing, Peoples R China

2.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Beijing, Peoples R China

3.Beijing Univ Agr, Coll Anim Sci & Technol, Beijing, Peoples R China

4.Tsinghua Univ, Ctr Stem Cell Biol & Regenerat Med, Sch Med, Beijing, Peoples R China

5.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci,State Key Lab Epigenet, Shanghai, Peoples R China

6.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Peoples R China

期刊名称：NATURE （ 影响因子：48.5； 五年影响因子：55.0 ）

ISSN： 0028-0836

年卷期： 2025 年

页码：

收录情况： SCI

摘要： After fertilization, early embryos undergo dissolution of conventional chromatin organization, including topologically associating domains (TADs)1,2. Zygotic genome activation then commences amid unusually slow de novo establishment of three-dimensional chromatin architecture2. How chromatin organization is established and how it interplays with transcription in early mammalian embryos remain elusive. Here we show that CTCF occupies chromatin throughout mouse early development. By contrast, cohesin poorly binds chromatin in one-cell embryos, coinciding with TAD dissolution. Cohesin binding then progressively increases from two- to eight-cell embryos, accompanying TAD establishment. Unexpectedly, strong 'genic cohesin islands' (GCIs) emerge across gene bodies of active genes in this period. GCI genes enrich for cell identity and regulatory genes, display broad H3K4me3 at promoters, and exhibit strong binding of transcription factors and the cohesin loader NIPBL at nearby enhancers. We show that transcription is hyperactive in two- to eight-cell embryos and is required for GCI formation. Conversely, induced transcription can also create GCIs. Finally, GCIs can function as insulation boundaries and form contact domains with nearby CTCF sites, enhancing both the transcription levels and stability of GCI genes. These data reveal a hypertranscription state in early embryos that both shapes and is fostered by the three-dimensional genome organization, revealing an intimate interplay between chromatin structure and transcription.