Transglutaminase 2 reduces the polyspermy of porcine oocytes by promoting the hardening of zona pellucida
文献类型： 外文期刊
作者： Cui, Zhaokang 1 ; Zhang, Jianli 1 ; Zhang, Jun 1 ; Zhong, Jifeng 1 ; Wang, Huili 1 ; Xiong, Bo 2 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China
2.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Peoples R China
关键词： Transglutaminase 2; Polyspermy; ZP3; ZP hardening; Porcine oocytes
期刊名称：THERIOGENOLOGY （ 影响因子：2.5； 五年影响因子：2.7 ）
ISSN： 0093-691X
年卷期： 2025 年 236 卷
页码：
收录情况： SCI
摘要： Polyspermy is a type of abnormal fertilization that would increase the risk of embryonic death and hence reduced female fecundity. To avoid this occurrence, mammalian oocytes develop the complicated mechanisms of blocks to polyspermy. Our previous study discovered that transglutaminase 2 (Tgm2) is a newly identified molecule responsible for the post-fertilization block to sperm penetration through zona pellucida (ZP) and polyspermy in mice. However, whether this function of Tgm2 is conserved across species has not been clarified. This study applied the porcine oocytes as a model to address this question. It was observed that inhibition of TGM2 activity by using its specific inhibitor LDN-27219 exhibited a substantially increased rate of polyploid zygotes after in vitro fertilization. In addition, our study validated that recombinant TGM2 protein interacted with zona pellucida glycoprotein 3 (ZP3) and crosslinked it to promote the ZP hardening. Further, incubation of porcine oocytes with recombinant TGM2 protein effectively reduced the occurrence of polyploid zygotes. Collectively, our data reveal that TGM2 performs a conserved function in porcine oocytes for zona block and polyspermy prevention.
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