文献类型： 外文期刊

作者： Song, Xuan ¹ ; Zhang, Da-wei ⁴ ; Yang, Yong ³ ; Kou, Jun-feng ⁶ ; Liu, Chang ⁷ ; Yi, Chao-qun ² ; Tang, Zi-yi ² ; Liu, Wen-tao ² ; Chen, Run ² ; Guo, Wei-hao ² ; Zhang, Tian-tao ² ; Zhang, Yong-jun ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Zhongyuan Res Ctr, Xinxiang 453500, Henan, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Zhangye Acad Agr Sci, Inst Plant Protect, Zhangye 734000, Gansu, Peoples R China

4.Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou 730070, Gansu, Peoples R China

5.Chinese Acad Agr Sci, Inst Plant Protect, Natl Key Lab Integrated Management Crop Dis & Pest, Beijing 100193, Peoples R China

6.CangZhou Acad Agr & Forestry Sci, Cangzhou 061000, Heibei, Peoples R China

7.Ningxia Acad Agr & Forestry Sci, Inst Plant Protect, Yinchuan 750002, Ningxia, Peoples R China

8.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China

9.Agr Univ Hebei, Coll Plant Protect, Baoding 071000, Hebei, Peoples R China

关键词： Monolepta hieroglyphica; Coleoptera pest; Juvenile hormone acid O-methyltransferase; Molecular dynamics; RNAi

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 318 卷

页码：

收录情况： SCI

摘要： Monolepta hieroglyphica, a major pest of Chinese crops, demands effective control. Targeting juvenile hormone acid O-methyltransferase, a key enzyme in JH production, presents a promising strategy for developing novel, specific insecticides. This study investigated the structure-function of M. hieroglyphica juvenile hormone acid Omethyltransferase (MhieJHAMT), a key enzyme in the JH pathway. Phylogenetic analysis showed MhieJHAMT's conservation within Coleoptera, suggesting a conserved catalytic mechanism. In vitro enzymatic activity assays confirmed MhieJHAMT's catalytic function. Bioinformatics (molecular dynamics and docking) revealed 19 amino acid residues forming the active site, binding S-adenosylmethionine (SAM) and farnesoic acid (FA). In vivo RNA interference (RNAi) coupled with ELISA demonstrated MhieJHAMT's crucial role in JH biosynthesis; its knockdown significantly reduced JH titers. Quantitative real-time PCR (RT-qPCR) revealed higher MhieJHAMT expression in female intestines and gonads, hinting at additional functions beyond JH biosynthesis. Interestingly, our findings reveal an intriguing correlation between host plant-mediated alterations in farnesyl pyrophosphate synthase 1 (MhieFPPS1) expression and that of MhieJHAMT, with MhieFPPS1 appearing to function upstream of MhieJHAMT. The observation suggests a potential positive feedback loop, hinting at a complex regulatory mechanism. This comprehensive study elucidates MhieJHAMT's function, its regulatory mechanisms, and its potential as a novel pest control target.