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Discovery of a new highly pathogenic toxin involved in insect sepsis

文献类型： 外文期刊

作者： Zhang, Yuan 1 ; Li, Hao 1 ; Wang, Fang 2 ; Liu, Chang 2 ; Reddy, Gadi V. P. 3 ; Li, Hu 1 ; Li, Zhihong 1 ; Sun, Yucheng 5 ; Zhao, Zihua 1 ;

作者机构： 1.China Agr Univ, Coll Plant Protect, MARA Key Lab Surveillance & Management Plant Quara, Beijing, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Inst Plant Protect, Yinchuan, Peoples R China

3.Lousiana State Univ, Dept Entomol, Los Angeles, CA USA

4.China Agr Univ, Sanya Inst, Sanya, Peoples R China

5.Chinese Acad Sci, Inst Zool, Beijing, Peoples R China

关键词： Steinernema; Xenorhabdus; phenotypic variation; apoptosis; Kyn pathway; actinomycin D

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Insect sepsis is a severe consequence that arises from the invasion of the hemocoel by symbionts of entomopathogenic nematodes and bacteria. In the present study, we unveiled the heightened virulence of the entomopathogenic nematode Steinernema feltiae and the entomopathogenic bacteria Xenorhabdus bovienii, which operate symbiotically, against the wax moth Galleria mellonella. Maximum mortality was observed at 25 degrees C while the optimal infestation efficiency was 20 nematodes per host. After infestation, G. mellonella displayed rapid darkening and softening, accompanied by an escalated esterase activity at 9 h. The X. bovienii, released by S. feltiae, underwent substantial proliferation and discharged toxins that attacked hemocytes, thus triggering extensive hemolysis and sepsis. The host G. mellonella was usually killed within 24 h due to disseminated septicemia. Additionally, X. bovienii infestation led to the upregulation of metabolites like 3-hydroxyanthranilic acid. Strikingly, we identified the perilous actinomycin D, generated through kynurenine metabolites, representing a novel biomarker of insect sepsis. Furthermore, a comprehensive transcriptomic analysis unveiled a noteworthy upregulation of gene expression associated with actinomycin D. Overall, X. bovienii induced apoptosis and sepsis through actinomycin D production, indicating its pivotal role in infestation activity. These findings open up new avenues for studying the mechanism of sepsis and developing innovative biotic pesticides.

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