Integrated genomic and transcriptomic analysis reveals the mechanisms underlying leaf variegation in 'Gonggan' mandarin
文献类型： 外文期刊
作者： Shi, Cong 1 ; Gu, Miaofeng 1 ; Huang, Yongjing 2 ; You, Congjun 1 ; Bao, Sihan 1 ; Xie, Shuangling 1 ; Gong, Jinli 1 ; Deng, Guiming 2 ; Wu, Pingzhi 2 ; Wu, Wen 2 ; Zhu, Congyi 2 ; Sun, Xuepeng 1 ; Zeng, Jiwu 2 ;
作者机构： 1.Zhejiang A&F Univ, Key Lab Qual & Safety Control Subtrop Fruit & Vege, Minist Agr & Rural Affairs, Hangzhou 311300, Zhejiang, Peoples R China
2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangdong Prov Key Lab Sci & Technol Res Fruit Tre, Guangzhou 510640, Peoples R China
关键词： Plant genome; leaf variegation; Chloroplast development; Citrus; Genome assembly; Transcriptome; Gene co-expression network
期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）
ISSN： 1471-2229
年卷期： 2025 年 25 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundThe 'Gonggan' mandarin, an elite local cultivar from Zhaoqing City, Guangdong Province, combines the qualities of mandarin and sweet orange. A leaf-variegated mutant enhances its ornamental and economic value, providing an excellent model for studying chloroplast development and photosynthetic pigment metabolism in citrus.ResultsWe found that, in this variegated mutant, chloroplasts are severely deficient or absent in mesophyll cells. Physiological assessments revealed lower levels of chlorophyll, carotenoids, net photosynthetic rate (Pn), and stomatal conductance (Gs), alongside significantly higher non-photochemical quenching (NPQ) and the non-photochemical quenching coefficient (qN), reflecting increased photoprotective energy dissipation. To uncover the molecular basis of leaf variegation, high-quality genome assemblies and transcriptomes were generated for both the normal and variegated 'Gonggan' mandarin, enabling comparative multi-omics analysis. Key genes involved in chloroplast development, such as TOC159, PDV2, THA8, and SIG5, were downregulated in the variegated leaves. Similarly, structural genes linked to chlorophyll degradation, including CLH2, SGR, NOL, and NYC1, exhibited altered expression. Downregulation of transcription factors GLK, GNC, and GNC-LIKE (GNL), known regulators of chloroplast development and chlorophyll biosynthesis, was also observed.ConclusionsThese findings suggest that disrupted expression of critical genes impacts chloroplast development and pigment metabolism, causing the leaf variegation phenotype. Overall, this study lays a foundation for functional genomics research and potential germplasm improvement of 'Gonggan' mandarin, and provides new insights into the mechanisms driving color variation in citrus.
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