Integrated untargeted metabolomic and transcriptomic analyses reveal OMT genes controlling polymethoxyflavonoid biosynthesis in the pericarp of Citrus reticulata 'Chachi'
文献类型： 外文期刊
作者： Li, Xinqi 1 ; Mao, Genlin 1 ; Chen, Wanbing 1 ; Wu, Pingzhi 1 ; Zhang, Ruimin 1 ; Zhang, Man 1 ; Huang, Yongjing 1 ; Xu, Juan 4 ; Zeng, Jiwu 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou, Peoples R China
3.Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou 510640, Peoples R China
4.Huazhong Agr Univ, Qual Anal Ctr Hort Prod, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Peoples R China
5.Hubei Hongshan Lab, Wuhan 430070, Peoples R China
关键词： Citrus functional qualities; Polymethoxyflavonoid; Potential biomarkers; Multi-omics combined analysis; Gene expression
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.3； 五年影响因子：4.5 ）
ISSN： 0304-4238
年卷期： 2024 年 327 卷
页码：
收录情况： SCI
摘要： The dried pericarp of Citrus reticulata 'Chachi' (CRC) is commonly utilized in Traditional Chinese Medicine (TCM) and food consumption. Geqingpi (GQP), Sihuaqingpi (SHQP) and Guangchenpi (CRP) are TCM materials derived from different developmental stages of CRC pericarp, each possessing distinct medicinal properties. However, there is currently limited study on the active substances in these three stages. Metabolome data suggested that the discrepancies in flavonoid content, particularly polymethoxyflavonoids (PMFs), predominantly contribute to the variations across the three periods. Additionally, through multivariate statistical analysis, 7 potential biomarkers were identified and nobiletin (NOB) possessed the most significant contribution. Omethyltransferases (OMTs) play a crucial role to catalyze the formation of -OCH3 in PMFs. The analysis of transcriptome and genome data revealed the presence of 93 CrOMTs in CRC and 43 were expressed on pericarp. Further investigation identified that 28 of these genes were potentially responsible for catalyzing the biosynthesis of important PMFs such as NOB, sinensetin (SIN), and 3,3 ',4 ',5,6,7,8-heptamethoxyflavone (HEP). Our results may provide a new perspective for the study of GQP, SHQP and CRP, as well as the regulatory mechanism of PMFs in CRC.
- 相关文献
作者其他论文 更多>>
-
A smartphone-based electrochemical sensing platform for the portable and simultaneous determination of flavonoids in Citri Reticulatae Pericarpium
作者：Xia, Hong-qi;Zhu, Congyi;Qiu, Diyang;Zeng, Jiwu
关键词：Portable electrochemical sensing platform; Laser induced graphene; Citri reticulatae pericarpium; Polymethoxylated flavones; Hesperidin
-
A newly isolated intestinal bacterium involved in the C-ring cleavage of flavan-3-ol monomers and the antioxidant activity of the metabolites
作者：Wang, Li;Liu, Ruonan;Yan, Fangfang;Zhang, Mo;Lu, Qun;Huang, Bijun;Liu, Rui;Wang, Li;Liu, Ruonan;Yan, Fangfang;Zhang, Mo;Lu, Qun;Huang, Bijun;Liu, Rui;Chen, Wanbing;Lu, Qun;Liu, Rui;Liu, Rui
关键词：
-
Flavor characterization of Citri Reticulatae Pericarpium (Citrus reticulata 'Chachiensis') with different aging years via sensory and metabolomic approaches
作者：Liu, Yuan;Wen, Huan;Kong, Jiatao;Hu, Zhehui;Zhang, Hongyan;Chen, Jiajing;Xu, Juan;Liu, Yuan;Wen, Huan;Kong, Jiatao;Hu, Zhehui;Zhang, Hongyan;Chen, Jiajing;Xu, Juan;Hu, Yang;Zeng, Jiwu;Chen, Xiangling
关键词：Guangchenpi (GCP); Aging process; Sensory evaluation; Flavor -related metabolites; Characteristic aroma compounds; Predictive modeling
-
Bruceine D inhibits the growth of Spodoptera litura by inducing cell apoptosis in the midgut via an oxidative burst
作者：Zeng, Jiwu;Mao, Genlin;Xu, Yuhui;Zeng, Jiwu;Lu, Wei;Xu, Yuhui;Liu, Huan;Xu, Hanhong;Xu, Hanhong
关键词：Bruceine D; Spodoptera litura; growth and development; midgut; ROS; apoptosis
-
Four novel Cit7GlcTs functional in flavonoid 7-O-glucoside biosynthesis are vital to flavonoid biosynthesis shunting in citrus
作者：Yuan, Ziyu;Li, Gu;Zhang, Huixian;Peng, Zhaoxin;Ding, Wenyu;Wen, Huan;Zhou, Hanxin;Chen, Jiajing;Xu, Juan;Yuan, Ziyu;Li, Gu;Zhang, Huixian;Chen, Jiajing;Xu, Juan;Zeng, Jiwu
关键词：
-
Combined analysis of GC-MS, RNA-seq and ATAC-seq elucidates the essential oils variation and terpenes biosynthesis in Citrus grandis 'Tomentosa'
作者：Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu;Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu;Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu
关键词：Essential oils; GC-MS; Transcriptome; ATAC-seq; Terpenes variations; Transcriptional factors
-
The gap-free genome and multi-omics analysis of Citrus reticulata 'Chachi' reveal the dynamics of fruit flavonoid biosynthesis
作者：Zhu, Congyi;Wu, Pingzhi;Huang, Yongjing;Zhang, Ruimin;Fan, Zhengyan;Zeng, Jiwu;You, Congjun;Yu, Chao;Gong, Jinli;Hu, Xiaoli;Sun, Xuepeng;You, Congjun;Yu, Chao;Gong, Jinli;Hu, Xiaoli;Sun, Xuepeng
关键词：
