Bruceine D inhibits the growth of Spodoptera litura by inducing cell apoptosis in the midgut via an oxidative burst
文献类型： 外文期刊
作者： Mao, Genlin 2 ; Lu, Wei 3 ; Xu, Yuhui 2 ; Liu, Huan 4 ; Xu, Hanhong 5 ; Zeng, Jiwu 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Fruit Tree Res Inst, Guangzhou 510640, Peoples R China
2.Guangdong Acad Agr Sci, Key Lab South Subtrop Fruit Biol & Genet Resource, Minist Agr & Rural Affairs, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou, Peoples R China
3.Xinjiang Agr Univ, Coll Agron, Urumqi, Peoples R China
4.Henan Univ Sci & Technol, Coll Hort & Plant Protect, Luoyang, Peoples R China
5.South China Agr Univ, Natl Key Lab Green Pesticide, Guangzhou, Peoples R China
6.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou, Peoples R China
关键词： Bruceine D; Spodoptera litura; growth and development; midgut; ROS; apoptosis
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）
ISSN： 1526-498X
年卷期： 2024 年
页码：
收录情况： SCI
摘要： BACKGROUND Spodoptera litura is one of the most harmful lepidoptera pests in China, and is difficult to control due to its strong resistance to the current frequently used insecticide species. The requirement to develop pesticides with novel toxicology mechanisms to control S. litura is urgent. The quassinoid of bruceine D display outstanding systemic properties and strong insecticidal activity against S. litura, which possess notable application potential for integrative management of S. litura, but the mechanism of toxicity remains unclear. RESULTS In this study, we found that bruceine D exerts potent growth inhibitory activity against S. litura, disrupting the ecdysone and juvenile hormone titers, and causing long-term adverse effects. Association analysis between transcriptomics and metabolomics suggested that bruceine D affected the digestion and absorption capacity of S. litura larvae by inducing a strong oxidative stress response and cell apoptosis in the intestine. Further analysis demonstrated that bruceine D can inhibit the activities of digestive and antioxidant enzymes and induce malondialdehyde (MDA) and reactive oxygen species (ROS) overaccumulation in the midgut. Moreover, the protein level of Bax, cleavage caspase 3, and cytochrome c expressed in cytoplasm (cyto) were up-regulated by bruceine D, while Bcl-2 and cytochrome c expressed in mitochondria (mito) were down-regulated. In addition, there was a noticeable increase in caspase-3 protease activity. Histopathological observations revealed that bruceine D damages the structure of midgut epithelial cells and activates lysosomes, which subsequently disrupts the midgut tissue. CONCLUSION Overall, our findings suggested that bruceine D induced excessive ROS accumulation in midgut epithelial cells. The resulting cell apoptosis disrupted midgut tissue, leading ultimately to reduced nutrient digestion and absorption in the midgut and the inhibition of larval growth. (c) 2024 Society of Chemical Industry.
- 相关文献
作者其他论文 更多>>
-
Integrated genomic and transcriptomic analysis reveals the mechanisms underlying leaf variegation in 'Gonggan' mandarin
作者：Shi, Cong;Gu, Miaofeng;You, Congjun;Bao, Sihan;Xie, Shuangling;Gong, Jinli;Sun, Xuepeng;Shi, Cong;Huang, Yongjing;Deng, Guiming;Wu, Pingzhi;Wu, Wen;Zhu, Congyi;Zeng, Jiwu
关键词：Plant genome; leaf variegation; Chloroplast development; Citrus; Genome assembly; Transcriptome; Gene co-expression network
-
Herbivore-induced maize volatiles: dual functions in repelling fall armyworm and attracting natural enemies
作者：Wang, Jiali;Yi, Ting;Wang, Manwen;Wei, Jiaqi;Yan, Wenjuan;Wen, Yingjie;Zeng, Lingda;Xu, Hanhong;Wang, Jiali;Wen, Yingjie
关键词：herbivore-induced plant volatiles (HIPVs); fall armyworm (FAW); natural enemy; oviposition inhibition; sustainable pest management
-
Guava root exudate-driven rhizosphere microorganisms changes transmitted to foliar-feeding insects influence their feeding behaviour
作者：Zhang, Peiwen;Shao, Xuehua;Zhang, Peiwen;Zhang, Zhixiang;Lu, Wei;Yue, Lei
关键词：guava; root exudates; rhizosphere microorganisms;
Spodoptera litura ; gut microorganisms -
Inhibition of Glucosinolate Sulfatases to Combat Plutella xylostella: Development of Novel Phenylcarboxamide Derivatives for Plant-Integrated Pest Management
作者：Wang, Jiali;Wang, Manwen;Wen, Yingjie;Zeng, Lingda;Xu, Hanhong;Ravi, Anil;Zaraei, Seyed-Omar;Alkubaisi, Bilal O.;El-Gamal, Mohammed I.;Wang, Jiali;Wen, Yingjie;Wen, Yingjie;El-Gamal, Mohammed I.;El-Gamal, Mohammed I.
关键词：
Plutella xylostella ; glucosinolatesulfatases; GSSs inhibitions; isothiocyanates; phenylcarboxamide derivatives; plant defense system -
The LUX-SWI3C module regulates photoperiod sensitivity in Arabidopsis thaliana
作者：Wang, Jianhao;Liu, Huan;Li, Hong;Wang, Fan;Yue, Lin;Liu, Shuangrong;Liu, Baohui;Kong, Fanjiang;Sun, Zhihui;Wang, Jianhao;Yang, Songguang;Huang, Mingkun
关键词：chromatin remodeling; circadian clock; evening complex; histone modification; photoperiod sensitivity
-
The Effects of Different Cotton Varieties on the Growth and Feeding Preferences of Helicoverpa armigera
作者：Yue, Lei;Dong, Jianhao;Xia, Guoyi;Yan, Xiang;Tuerxun, Aizimaitijiang;Lu, Wei;Yue, Lei;Dong, Jianhao;Xia, Guoyi;Yan, Xiang;Tuerxun, Aizimaitijiang;Lu, Wei;Shao, Xuehua;Shao, Xuehua;Lu, Wei
关键词：cotton pest; feeding area; developmental duration; fecundity;
Helicoverpa armigera -
Insights into the toxicity effects of indoxacarb against Spodoptera frugiperda using metabolomics combined with mass spectrometry imaging
作者：Ma, Lianlian;Tang, Yinyi;Yang, Shuai;Li, Zhen;Zhang, Zhixiang;Xu, Hanhong;Wu, Xinzhou;Ma, Lianlian;Shao, Xuehua
关键词：indoxacarb; Spodoptera frugiperda; spatial metabolomics; toxicity effects; mass spectrometry imaging
