Influence of Two Hexose Transporters on Substrate Affinity and Pathogenicity in Magnaporthe oryzae
文献类型： 外文期刊
作者： Huang, Tinghong 1 ; Guo, Dekang 1 ; Luo, Xiao 1 ; Chen, Ronghua 1 ; Wang, Wenjuan 2 ; Xu, Hanhong 1 ; Chen, Shen 2 ; Lin, Fei 1 ;
作者机构： 1.South China Agr Univ, Natl Key Lab Green Pesticide, Key Lab Nat Pesticide & Chem Biol, Minist Educ, Guangzhou 510642, Peoples R China
2.Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510642, Peoples R China
关键词： hexose transporters; pathogenicity; substrate affinity; Magnaporthe oryzae
期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）
ISSN：
年卷期： 2024 年 12 卷 4 期
页码：
收录情况： SCI
摘要： Hexose transporters (HXT) play a crucial role in the pathogenicity of Magnaporthe oryzae, serving not only as key facilitators for acquiring and transporting sugar nutrients to support pathogen development, but also as sugar sensors which receive transduction signals. The objective of this study is to investigate the impact of MoHXT1-3 on rice pathogenicity and hexose affinity. MoHXT1-3 deletion mutants were generated using CRISPR/Cas9 technology, and their affinity for hexose was evaluated through yeast complementation assays and electrophysiological experiments in Xenopus oocytes. The results suggest that MoHXT1 does not contribute to melanin formation or hexose transportation processes. Conversely, MoHXT2, despite displaying lower affinity towards the hexoses tested in comparison to MoHXT3, is likely to have a more substantial impact on pathogenicity. The analysis of the transcription profiles demonstrated that the deletion of MoHXT2 caused a decrease in the expression of MoHXT3, whereas the knockout of MoHXT3 resulted in an upregulation of MoHXT2 transcription. It is noteworthy that the MoHXT2(M145K) variant displayed an incapacity to transport hexoses. This investigation into the functional differences in hexose transporters in Magnaporthe oryzae provides insights into potential advances in new strategies to target hexose transporters to combat rice blast by blocking carbon nutrient supply.
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