文献类型： 外文期刊

作者： Ma, Zhenbao ¹ ; Zeng, Zhenling ² ; Liu, Jiao ² ; Liu, Chang ² ; Pan, Yu ² ; Zhang, Yanan ⁴ ; Li, Yafei ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Qual Standard & Monitoring Technol Agroprod, Guangzhou 510640, Peoples R China

2.South China Agr Univ, Guangdong Prov Key Lab Vet Pharmaceut Dev & Safet, Guangzhou 510642, Peoples R China

3.South China Agr Univ, Natl Risk Assessment Lab Antimicrobial Resistance, Guangzhou 510642, Peoples R China

4.Guizhou Acad Agr Sci, Inst Anim Husb & Vet Med, Guiyang 550005, Peoples R China

关键词： Escherichia coli; bla (NDM-5); IncHI2 plasmid; resistance

期刊名称：PATHOGENS （ 影响因子：3.492； 五年影响因子：4.066 ）

ISSN：

年卷期： 2021 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Carbapenem resistance has posed potential harmful risks to human and animals. The objectives of this study were to understand the prevalence of bla(NDM-5) in pigs and investigate the molecular characteristics of NDM-5-producing Escherichia coli isolates in Guangdong province in China. Carbapenem-resistant E. coli isolates were isolated from pigs and obtained using MacConkey plates containing 0.5 mg/L meropenem. Conjugation assay and antimicrobial susceptibility testing were conducted for the isolates and their transconjugants. Whole-genome sequence (WGS) was used to analyze the plasmid genetic feature. A total of five bla(NDM-5)-carrying E. coli isolates were obtained in the present investigations. They belonged to five ST types. The bla(NDM-5) genes were found to be in IncX3 and IncHI2 plasmid. The IncX3 plasmid was 46,161 bp in size and identical to other reports. IncHI2 plasmid was 246,593 bp in size and similar to other IncHI2-ST3 plasmids. It consisted of a typical IncHI2 plasmid backbone region and a multiresistance region (MRR). The bla(NDM- 5) was closely associated with the IS3000-ISAba125-blaNDM-5-bleMBL-trpF-tat-IS26 unit. We first reported the bla(NDM-5)-carrying IncHI2 in E. coli isolates recovered from pigs and revealed the molecular characterization. Continued surveillance for the dissemination of bla(NDM-5) among food-producing animals is required.