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MiR-214 inhibits NF-κB pathway activation to alleviate lipopolysaccharide-induced mastitis by targeting TRAF1

文献类型： 外文期刊

作者： Gao, Yujin 2 ; Li, Cheng 3 ; Zhang, Xijun 2 ; Yao, Dan 2 ; Zhang, Yong 1 ;

作者机构： 1.Longhu Lab Adv Immunol, Zhengzhou 450046, Peoples R China

2.Gansu Key Lab Anim Generat Physiol & Reprod Regula, Lanzhou 730070, Peoples R China

3.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China

关键词： Dairy cow mastitis; miR-214; NF-kappa B; TRAF1

期刊名称：THERIOGENOLOGY （ 影响因子：2.5； 五年影响因子：2.7 ）

ISSN： 0093-691X

年卷期： 2025 年 237 卷

页码：

收录情况： SCI

摘要： Mastitis in dairy cows is defined by inflammation of mammary tissue, and represents a significant challenge in the dairy industry. The microRNA miR-214 is recognized as a key endogenous regulatory molecule with a critical role in inflammatory diseases. However, its involvement in the regulation of mastitis remains unclear. This study, investigated the role of miR-214 in dairy mastitis and explored its therapeutic potential. It was observed that miR-214 expression was reduced in an in vivo lipopolysaccharide (LPS)-induced mouse mastitis model and an in vitro LPS-induced bovine mammary epithelial cell (bMEC) inflammation model. The miR-214 mimic was found to suppress the expression of inflammatory cytokines IL-1 beta, TNF-alpha, and IL-6. Furthermore, the miR-214 mimic inhibited nuclear factor-kappa B (NF-kappa B) pathway activation in LPS-induced bMECs. Dual-luciferase reporter assay results confirmed that miR-214 targeted tumor necrosis factor receptor-associated factor 1 (TRAF1) to inhibit its expression. Silencing TRAF1 in bMECs reduced LPS-induced expression of inflammatory cytokines and NF-kappa B pathway activation. Conversely, TRAF1 overexpression negated the inhibitory effects of miR-214 on LPS-induced inflammatory cytokines expression and NF-kappa B pathway activation in bMECs. Additionally, in the in vivo LPSinduced mouse mastitis model, miR-214 alleviated pathological damage and decreased inflammatory cytokines expression in mammary tissue. These findings suggest that miR-214 inhibits NF-kappa B activation by downregulating TRAF1 expression thereby mitigating LPS-induced inflammatory responses. This study highlights a potential novel approach for the treatment of mastitis in dairy cows.

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