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Characterization Studies on the sugC Gene of Streptococcus suis Serotype 2 in Adhesion, Invasion, and Virulence in Mice

文献类型： 外文期刊

作者： Dong, Zhimin 1 ; Li, Cheng 1 ; Tian, Xiangxue 1 ; Guo, Xiaoran 1 ; Li, Xiuli 3 ; Ren, Weike 1 ; Chi, Jingjing 1 ; Zhang, Li 1 ; Li, Fuqiang 1 ; Zhu, Yao 4 ; Zhang, Wanjiang 4 ; Yan, Minghua 1 ;

作者机构： 1.Tianjin Acad Agr Sci, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin Engn Res Ctr Anim Hlth Farming, Inst Anim Sci & Vet, Tianjin 300381, Peoples R China

2.Natl Data Ctr Anim Hlth, Tianjin 300381, Peoples R China

3.Tianjin Acad Agr Sci, Inst Agroprod Safety & Nutr, Tianjin 300381, Peoples R China

4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Harbin 150069, Peoples R China

关键词： Streptococcus suis serotype 2; sugC gene; adhesion; invasion; pathogenicity

期刊名称：VETERINARY SCIENCES （ 影响因子：2.3； 五年影响因子：2.4 ）

ISSN：

年卷期： 2024 年 11 卷 9 期

页码：

收录情况： SCI

摘要： The sugC gene of Streptococcus suis (S. suis) is a coding gene for the ATP-binding transporter-associated protein with strong pathogenicity. In order to reveal the effect of the sugC gene on the virulence of S. suis serotype 2, a wild-type strain of TJS75, isolated from fattening pigs' brain tissue samples, was used as a parent strain, and a knockout sugC gene (Delta sugC) and complementary strain (C Delta sugC) were successfully constructed via homologous recombination technology. The biological characteristics of TJS75, Delta sugC and C Delta sugC were compared and analyzed through growth curves, biochemical characteristics, hemolysis characteristics, cell infection tests and pathogenicity tests on BALB/c mice. The results of the growth characteristic experiments in vitro showed that the plateau stage growth period of Delta sugC was delayed compared to the TJS75 strain, but there was no difference in the total number of bacteria. The biochemical characteristics and hemolysis ability of Delta sugC in sheep blood had no difference compared with TJS75, but its adhesion and invasion abilities in PK-15 cells were decreased. Knockout of the sugC gene had no impact on the expression levels of adhesion-related genes in TJS75 in real-time PCR analysis. In addition, the LD50 of Delta sugC in BALB/c mice was 1.47 x 10(8) CFU, seven times higher than that of TJS75 (LD50 = 2.15 x 10(7) CFU). These results illustrate that the deletion of sugC reduced the virulence of TJS75 to BALB/c mice, but its role in the adhesion and invasion of PK-15 cells in this strain needs to be further explored. In summary, this study provides evidence that the sugC gene is a virulence-related gene in the S. suis serotype 2 strain and plays a crucial role in the adhesion and invasion of S. suis. This study lays a foundation for the further exploration of the potential virulence factors and pathogenesis of S. suis.

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