A novel HPLC-PDA method for judging chicken meat infected with Salmonella Enteritidis by simultaneous determination of four key
文献类型： 外文期刊
作者： Xu, Junjie 1 ; Ding, Hao 2 ; Chen, Lan 2 ; Cao, Yang 1 ; Hong, Yiwei 1 ; Guo, Yawen 1 ; Gao, Pengfei 2 ; Hong, Lu 1 ; Cai, Jingjing 1 ; Cheng, Sihui 1 ; Pang, Maoda 4 ; Guo, Xiaodong 1 ; Zhang, Tao 1 ; Zhang, Genxi 1 ; Xie, Kaizhou 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China
2.Yangzhou Univ, Joint Int Res Lab Agr & Agri Prod Safety, Yangzhou 225009, Peoples R China
3.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China
关键词： Chicken meat; Salmonella Enteritidis; Metabolite biomarkers; HPLC-PDA; L-proline; L-valine; L-methionine; L-leucine
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 2023影响因子：4.0； 五年影响因子：4.1 ）
ISSN： 0889-1575
年卷期： 2024 年 133 卷
收录情况： SCI
摘要： Detection of foodborne pathogen infection by detecting key metabolic markers is an effective measure to maintain food hygiene and safety. Therefore, the rapid, efficient, sensitive and convenient method of highperformance liquid chromatography-photodiode array (HPLC-PDA) was established and optimized to determine four key metabolic biomarkers in chicken meat infected with Salmonella Enteritidis, including L-proline (Pro), L-valine (Val), L-methionine (Met), L-leucine (Leu). The limits of detection and quantification (LOD, LOQ) for four target metabolic biomarkers were 4.50 -6.5 mu g/kg and 25 mu g/kg, respectively. The calibration curves show a good linear relationship (R 2 >= 0.9996). The recoveries were in the range of 80 -120 %, and the relative standard deviations (RSD) were all lower than 6 %. The experimental results can be used for rapid detection of chicken meat contaminated by Salmonella Enteritidis to ensure food safety. Furthermore, the HPLC-PDA method is well applied in practice.
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