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A Novel Polymer Nanoparticle Polydimethyl Diallyl Ammonium Chloride as An Adjuvant Enhances the Immune Response of SARS-CoV-2 Subunit Vaccine

文献类型： 外文期刊

作者： Ren, Lili 1 ; Ouyang, Chengcheng 1 ; Zhao, Shuqing 1 ; Zheng, Qiqi 1 ; Guo, Weilu 1 ; Fan, Baochao 2 ; Zhou, Jinzhu 2 ; Zhang, Wei 1 ; Hu, Mi 2 ; Li, Jizong 2 ; Li, Bin 2 ;

作者机构： 1.Nanjing Tech Univ, Sch Pharm, Nanjing 211816, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol,K, Nanjing 210014, Peoples R China

3.Jiangsu Univ, Inst Life Sci, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

4.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China

5.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

关键词： adjuvant; polydimethyl diallyl ammonium chloride; SARS-CoV-2; subunit vaccine

期刊名称：ADVANCED HEALTHCARE MATERIALS （ 影响因子：10.0； 五年影响因子：10.6 ）

ISSN： 2192-2640

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The Coronavirus Disease 2019 (COVID-19) pandemic caused by SARS-CoV-2 has a significant impact on global health and the economy. It has underscored the urgent need for a stable, easily produced and effective vaccine. This study presents a novel approach using SARS-CoV-2 spike (S) protein-conjugated nanoparticles (NPs) in combination with cyclic GMP-AMP (cGAMP) (S-NPs-cGAMP) as a subunit vaccine. When mice are immunized, the antiserum of S-NPs-cGAMP group exhibits a 16-fold increase in neutralizing activity against a pseudovirus, compared to S protein group. Additionally, S-NPs-cGAMP induces even higher levels of neutralizing antibodies. Remarkably, the vaccine also triggers a robust humoral immune response, as evidenced by a notable elevation in virus-specific IgG and IgM antibodies. Furthermore, after 42 days of immunization, there is an observed increase in specific immune cell populations in the spleen. CD3+CD4+ and CD3+CD8+T lymphocytes, as well as B220+CD19+ and CD3-CD49b+ NK lymphocytes, show an upward trend, indicating a positive cellular immune response. Moreover, the S-NPs-cGAMP demonstrates promising results against the Delta strain and exhibits good cross-neutralization potential against other variants. These findings suggest that pDMDAAC NPs is potential adjuvant and could serve as a versatile platform for future vaccine development. The study develops a novel subunit vaccine (S-NPs-cGAMP) delivered by the polymer polydimethyl diallyl ammonium chloride (pDMDAAC) with SARS-CoV-2 S protein combined with cGAMP adjuvant. The S-NPs-cGAMP induces more robust immune response in mice, compared to SARS-CoV-2 S protein. These findings suggest that pDMDAAC has the immunopotentiating properties and could be a potential adjuvant. image

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