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Fabrication of nitrogen-doped graphene quantum dots based fluorescent probe and its application for simultaneous, sensitive and selective detection of umami amino acids

文献类型： 外文期刊

作者： Han, Tingting 1 ; Huang, Yang 1 ; Gao, Tianyi 3 ; Xia, Chunyue 1 ; Sun, Chong 1 ; Xu, Weimin 1 ; Wang, Daoying 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Cold Chain Logist Technol Agroprod, Nanjing 210014, Peoples R China

3.Suzhou Ind Pk Biotech Dev Co Ltd, Suzhou 215123, Peoples R China

关键词： Fluorescent probe; Nitrogen -doped graphene quantum dots; Simultaneous detection; L -glutamic acid; L -aspartic acid

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 404 卷

页码：

收录情况： SCI

摘要： In this work, nitrogen-doped graphene quantum dots (N-GQDs) were synthesized by an efficient one-step hy-drothermal using hydrated citric acid and urea as the carbon and nitrogen sources, respectively. The fluorescent quantum yield of the N-GQDs was up to 88.20 %, and the fluorescence lifetime was 12.29 ns. Based on the excellent fluorescence property, water-soluble and low cytotoxicity of N-GQDs, a novel fluorescent probe was fabricated and used for simultaneous detection of L-glutamic acid (L-Glu) and L-aspartic acid (L-Asp). Herein, Al3+ was used as a chelant agent for L-Glu shielding. The sensitive response of the probe towards L-Glu and L-Asp presented a wide concentration range with a low detection limit (S/N = 3). The fluorescent probe showed good selectivity, high stability and acceptable reproducibility in the application of L-Glu/L-Asp simultaneous detection in chicken soup. Meanwhile, the detection results of the fluorescent probe were correlated well with those ob-tained by amino acid analyzer.

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