文献类型： 外文期刊

作者： Yu, Jie ¹ ; Lei, Shaolin ² ; Fang, Shiting ¹ ; Tai, Niufang ¹ ; Yu, Wei ¹ ; Yang, Ziwei ¹ ; Gu, Lei ¹ ; Wang, Hongcheng ¹ ; Du, Xuye ¹ ; Zhu, Bin ¹ ; Cai, Mengxian ¹ ;

作者机构： 1.Guizhou Normal Univ, Sch Life Sci, Guiyang 550025, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Inst Oil Crops, Guiyang 550009, Peoples R China

关键词： Brassica oleracea; radish; interspecific hybridization; unexpected hybrids; chromosome behaviour

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 9 期

页码：

收录情况： SCI

摘要： Interspecific hybridization and accompanying backcross between crops and relatives have been recognized as a powerful method to broaden genetic diversity and transfer desirable adaptive traits. Crosses between radish (Raphanus sativus, RR, 2n = 18) and Brassica oleracea (CC, 2n = 18), which formed allotetraploid Raphanobrassica (RRCC, 2n = 36), initiated the construction of resynthetic allopolyploids. However, these progenies from the backcrosses between Raphanobrassica and the two diploid parents have not been well deciphered. Herein, thousands of backcrosses using both Raphanobrassica and the two diploid parents as pollen donors were employed. Several hybrids with expected (2n = 27) and unexpected chromosome numbers (2n = 26 and 2n = 36) were obtained. Fluorescence in situ hybridization (FISH) analysis with R-genome-specific sequences as probes demonstrated that the genome structures of the two expected hybrids were RRC and CCR, and the genome structures of the three unexpected hybrids were RRRC, CCCR, and RRC' (harbouring an incomplete C genome). The unexpected hybrids with extra R or C genomes showed similar phenotypic characteristics to their expected hybrids. FISH analysis with C-genome-specific sequences as probes demonstrated that the unexpected allotetraploid hybrids exhibited significantly more intergenomic chromosome pairings than the expected hybrids. The expected and unexpected hybrids provide not only novel germplasm resources for the breeding of radish and B. oleracea but also very important genetic material for genome dosage analysis.