Identification of a major QTL and candidate genes analysis for branch angle in rapeseed (Brassica napus L.) using QTL-seq and RNA-seq
文献类型： 外文期刊
作者： Lei, Shaolin 1 ; Chen, Li 2 ; Liang, Fenghao 1 ; Zhang, Yuling 1 ; Zhang, Chao 1 ; Xiao, Huagui 1 ; Tang, Rong 1 ; Yang, Bin 1 ; Wang, Lulu 1 ; Jiang, Huanhuan 1 ;
作者机构： 1.Guizhou Acad Agr Sci, Guizhou Oil Crops Res Inst, Guiyang, Guizhou, Peoples R China
2.Guizhou Rapeseed Res Inst, Guizhou Acad Agr Sci, Guiyang, Guizhou, Peoples R China
关键词： rapeseed; branch angle; BSA-seq; QTL mapping; RNA-Seq
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Introduction: Branching angle is an essential trait in determining the planting density of rapeseed (Brassica napus L.) and hence the yield per unit area. However, the mechanism of branching angle formation in rapeseed is not well understood. Methods: In this study, two rapeseed germplasm with extreme branching angles were used to construct an F-2 segregating population; then bulked segregant analysis sequencing (BSA-seq) and quantitative trait loci (QTL) mapping were utilized to localize branching anglerelated loci and combined with transcriptome sequencing (RNA-seq) and quantitative real-time PCR (qPCR) for candidate gene mining Results and discussion: A branching angle-associated quantitative trait loci (QTL) was mapped on chromosome C3 (C3: 1.54-2.65 Mb) by combining BSA-seq as well as traditional QTL mapping. A total of 54 genes had SNP/Indel variants within the QTL interval were identified. Further, RNA-seq of the two parents revealed that 12 of the 54 genes were differentially expressed between the two parents. Finally, we further validated the differentially expressed genes using qPCR and found that six of them presented consistent differential expression in all small branching angle samples and large branching angles, and thus were considered as candidate genes related to branching angles in rapeseed. Our results introduce new candidate genes for the regulation of branching angle formation in rapeseed, and provide an important reference for the subsequent exploration of its formation mechanism.
- 相关文献
作者其他论文 更多>>
-
Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks
作者：Chang, Yuguang;Zeng, Tao;Sun, Hanxue;Tian, Yong;Cao, Yongqing;Xu, Ligen;Duan, Mingcai;Lu, Lizhi;Chen, Li;Guo, Rongbing;Han, Xue
关键词：duck; ovary; RNA-seq; egg production
-
Integrating GWAS, RNA-Seq and functional analysis revealed that BnaA02.SE mediates silique elongation by affecting cell proliferation and expansion in Brassica napus
作者：Zhang, Liyuan;Li, Xiaodong;Chen, Si;Xiang, Sirou;Sun, Tingting;Yang, Ziyan;Kong, Xizeng;Qu, Cunmin;Li, Jiana;Yang, Bo;Zhang, Chao;Lu, Kun
关键词：Brassica napus; silique elongation; GWAS; transcriptome analysis; BnaA02.SE; functional research
-
Comprehensive high-throughput sequencing, evolutionary and functional analyses reveal the conservation and diversification of miR166s in regulating pleiotropic traits between rapeseed and Arabidopsis
作者：Zhang, Liyuan;Chen, Si;Yang, Ziyan;Xu, Jinxiong;Sun, Tingting;Kong, Xizeng;Qu, Cunmin;Li, Jiana;Zhang, Liyuan;Lu, Kun;Zhang, Chao;Yang, Bo;Kang, Lin
关键词：High -throughput sequencing; Pleiotropic traits; MiR166/HD-Zip III; Functional research; Rapeseed and Arabidopsis
-
Genome-wide identification and stress response analysis of BcaCPK gene family in amphidiploid Brassica carinata
作者：Zuo, Dan;Qian, Fang;Gu, Lei;Wang, Hongcheng;Du, Xuye;Zeng, Tuo;Zhu, Bin;Lei, Shaolin
关键词：Brassica carinata; Genome-wide; Calcium-dependent protein kinases (CPKs); Bioinformatics analysis; Stresses
-
Comprehensive analysis of the metabolome and the whole genome transcriptome to reveal the regulatory mechanism the flavor of Phallus rubrovolvatus
作者：Wang, Jian;Wang, Jian;Yang, Chenglong;Wang, Jian;Tian, Haoyuan;Xu, Yanjun;Peng, Xingcan;Zhang, Chao;Lu, Meilin;Wen, Tingchi;Wang, Jian;Zhang, Chao;Lu, Meilin;Wen, Tingchi;Tian, Haoyuan;Zhang, Chao;Peng, Xingcan;Lu, Zhenghua;Wen, Tingchi;Lu, Zhenghua;Xu, Shunyi
关键词：Phallus rubrovolvatus; Widely-targeted metabolome; Edible fungi; Amino acids; Flavor formation
-
Linkage Mapping and Discovery of Candidate Genes for Drought Tolerance in Rice During the Vegetative Growth Period
作者：Jiao, Aixia;Jiao, Aixia;Ma, Xiaoding;Cui, Di;Han, Bing;Han, Longzhi;Ma, Xiaoding;Cui, Di;Han, Bing;Han, Longzhi;Chen, Li;Ma, Jing;Sun, Jianchang
关键词：Rice; Drought tolerance; QTL; Candidate gene; Haplotype
-
Genome-wide identification of SIMILAR to RCD ONE (SRO) gene family in rapeseed (Brassica napus L.) reveals their role in drought stress response
作者：Jiang, Huanhuan;Zhang, Yuling;Li, Jia;Liang, Fenghao;Tang, Rong;Zhou, Yuyu;Zhang, Chao;Tang, Rongzi
关键词：Rapeseed; similar to RCD1 (SRO); abiotic stress; gene expression; transcription factor
