Comprehensive high-throughput sequencing, evolutionary and functional analyses reveal the conservation and diversification of miR166s in regulating pleiotropic traits between rapeseed and Arabidopsis
文献类型： 外文期刊
作者： Zhang, Liyuan 1 ; Zhang, Chao 3 ; Yang, Bo 4 ; Chen, Si 1 ; Yang, Ziyan 1 ; Kang, Lin 5 ; Xu, Jinxiong 1 ; Sun, Tingting 1 ; Kong, Xizeng 1 ; Qu, Cunmin 1 ; Lu, Kun 2 ; Li, Jiana 1 ;
作者机构： 1.Southwest Univ, Coll Agron & Biotechnol, Chongqing 400716, Peoples R China
2.Minist Educ, Engn Res Ctr South Upland Agr, Chongqing 400715, Peoples R China
3.Guizhou Acad Agr Sci, Oil Res Inst, Guiyang 550000, Peoples R China
4.Yunnan Acad Agr Sci, Ind Crops Res Inst, Kunming 650205, Peoples R China
5.Zhangjiakou Acad Agr Sci, Zhangjiakou 075000, Peoples R China
关键词： High -throughput sequencing; Pleiotropic traits; MiR166/HD-Zip III; Functional research; Rapeseed and Arabidopsis
期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）
ISSN： 0926-6690
年卷期： 2024 年 218 卷
页码：
收录情况： SCI
摘要： Rapeseed yield is quantitatively controlled by polygenes and multiple environmental factors, it is difficult to regulate pleiotropic characteristics via a single gene. MicroRNAs (miRNAs) are considered fine -tuners owning broad impacts on complex traits. However, numerous studies have focused only on screening miRNAs that regulate a single trait, and no miRNAs have been cloned and proven to be key regulators controlling pleiotropic characteristics in rapeseed. Here, high -throughput sequencing analysis both revealed three differentially expressed miRNAs among extreme materials regards to silique length (SL) and harvest index (HI). Among them, bna-miR166f was the main candidate miRNAs expressed differentially among multiple -yield cultivars. Then, transgenic experience with heterologous expression (overexpression and knockdown) confirmed that bnamiR166f regulates pleiotropic traits, including flowering time, silique development and branch numbers by targeting to all the five HD -Zip IIIs in Arabidopsis. However, based on the transgenic validation of bna-miR166f in Arabidopsis and plentiful miRNA-Seq data among yield -related cultivars in rapeseed, it was found that partially opposite phenotypic traits (especially SL) are exhibited between Arabidopsis and rapeseed. Joint miRNA/mRNASeq analysis revealed no negative expression profiles between bna-miR166f and the 17 putative BnaHD-Zip IIIs. Phylogenetic analysis revealed high evolutionary conservation of miR166, while the HD -Zip family experienced severe genome triplication in rapeseed, and the partial functional diversity between Arabidopsis and rapeseed may be caused by evolutionary divergence of the targets. Taken together, these results revealed the pivotal role of bna-miR166f in the genetic control of pleiotropic characteristics, thereby providing a theoretical basis for molecular breeding to improve rapeseed yield in the future.
- 相关文献
作者其他论文 更多>>
-
Integrating GWAS, RNA-Seq and functional analysis revealed that BnaA02.SE mediates silique elongation by affecting cell proliferation and expansion in Brassica napus
作者：Zhang, Liyuan;Li, Xiaodong;Chen, Si;Xiang, Sirou;Sun, Tingting;Yang, Ziyan;Kong, Xizeng;Qu, Cunmin;Li, Jiana;Yang, Bo;Zhang, Chao;Lu, Kun
关键词：Brassica napus; silique elongation; GWAS; transcriptome analysis; BnaA02.SE; functional research
-
Identification of a major QTL and candidate genes analysis for branch angle in rapeseed (Brassica napus L.) using QTL-seq and RNA-seq
作者：Lei, Shaolin;Liang, Fenghao;Zhang, Yuling;Zhang, Chao;Xiao, Huagui;Tang, Rong;Yang, Bin;Wang, Lulu;Jiang, Huanhuan;Chen, Li
关键词：rapeseed; branch angle; BSA-seq; QTL mapping; RNA-Seq
-
Comprehensive analysis of the metabolome and the whole genome transcriptome to reveal the regulatory mechanism the flavor of Phallus rubrovolvatus
作者：Wang, Jian;Wang, Jian;Yang, Chenglong;Wang, Jian;Tian, Haoyuan;Xu, Yanjun;Peng, Xingcan;Zhang, Chao;Lu, Meilin;Wen, Tingchi;Wang, Jian;Zhang, Chao;Lu, Meilin;Wen, Tingchi;Tian, Haoyuan;Zhang, Chao;Peng, Xingcan;Lu, Zhenghua;Wen, Tingchi;Lu, Zhenghua;Xu, Shunyi
关键词：Phallus rubrovolvatus; Widely-targeted metabolome; Edible fungi; Amino acids; Flavor formation
-
Genome-wide identification of SIMILAR to RCD ONE (SRO) gene family in rapeseed (Brassica napus L.) reveals their role in drought stress response
作者：Jiang, Huanhuan;Zhang, Yuling;Li, Jia;Liang, Fenghao;Tang, Rong;Zhou, Yuyu;Zhang, Chao;Tang, Rongzi
关键词：Rapeseed; similar to RCD1 (SRO); abiotic stress; gene expression; transcription factor
-
Transcriptome and Small RNA Sequencing Reveal the Mechanisms Regulating Harvest Index in Brassica napus
作者：Zhang, Chao;Chang, Wei;Li, Xiaodong;Yang, Bo;Zhang, Liyuan;Xiao, Zhongchun;Li, Jiana;Lu, Kun;Zhang, Chao;Chang, Wei;Li, Xiaodong;Yang, Bo;Zhang, Liyuan;Xiao, Zhongchun;Li, Jiana;Lu, Kun;Zhang, Chao;Li, Jiana;Lu, Kun
关键词：harvest index; transcriptome; miRNA; regulatory network; Brassica napus
-
BnKAT2 Positively Regulates the Main Inflorescence Length and Silique Number in Brassica napus by Regulating the Auxin and Cytokinin Signaling Pathways
作者：Yuan, Dashuang;Wang, Zhen;Qu, Cunmin;Zhu, Dongming;Wan, Huafang;Liang, Ying;Yuan, Dashuang;Wang, Zhen;Qu, Cunmin;Zhu, Dongming;Wan, Huafang;Liang, Ying;Yuan, Dashuang;Wang, Zhen;Qu, Cunmin;Zhu, Dongming;Wan, Huafang;Liang, Ying;Zhang, Yin
关键词：Brassica napus; BnKAT2; length of main inflorescence; silique number
-
Identification of candidate genes regulating seed oil content by QTL mapping and transcriptome sequencing in Brassica napus
作者：Xiao, Zhongchun;Zhang, Chao;Qu, Cunmin;Wei, Lijuan;Zhang, Liyuan;Yang, Bo;Lu, Kun;Li, Jiana
关键词：Brassica napus; seed oil content; QTL mapping; differentially expressed genes (DEGs); candidate genes
