文献类型： 外文期刊

作者： Yuan, Dashuang ¹ ; Zhang, Yin ⁴ ; Wang, Zhen ¹ ; Qu, Cunmin ¹ ; Zhu, Dongming ¹ ; Wan, Huafang ¹ ; Liang, Ying ¹ ;

作者机构： 1.Southwest Univ, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China

2.Southwest Univ, Acad Agr Sci, Chongqing 400715, Peoples R China

3.Chongqing Engn Res Ctr Rapeseed, Chongqing 400715, Peoples R China

4.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang 550018, Peoples R China

关键词： Brassica napus; BnKAT2; length of main inflorescence; silique number

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 13 期

页码：

收录情况： SCI

摘要： Brassica napus is the dominant oil crop cultivated in China for its high quality and high yield. The length of the main inflorescence and the number of siliques produced are important traits contributing to rapeseed yield. Therefore, studying genes related to main inflorescence and silique number is beneficial to increase rapeseed yield. Herein, we focused on the effects of BnKAT2 on the main inflorescence length and silique number in B. napus. We explored the mechanism of BnKAT2 increasing the effective length of main inflorescence and the number of siliques through bioinformatics analysis, transgenic technology, and transcriptome sequencing analysis. The full BnKAT2(BnaA01g09060D) sequence is 3674 bp, while its open reading frame is 2055 bp, and the encoded protein comprises 684 amino acids. BnKAT2 is predicted to possess two structural domains, namely KHA and CNMP-binding domains. The overexpression of BnKAT2 effectively increased the length of the main inflorescence and the number of siliques in B. napus, as well as in transgenic Arabidopsis thaliana. The type-A Arabidopsis response regulator (A-ARR), negative regulators of the cytokinin, are downregulated in the BnKAT2-overexpressing lines. The Aux/IAA, key genes in auxin signaling pathways, are downregulated in the BnKAT2-overexpressing lines. These results indicate that BnKAT2 might regulate the effective length of the main inflorescence and the number of siliques through the auxin and cytokinin signaling pathways. Our study provides a new potential function gene responsible for improvement of main inflorescence length and silique number, as well as a candidate gene for developing markers used in MAS (marker-assisted selection) breeding to improve rapeseed yield.