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Comparative transcriptomic analysis of seed coats with high and low lignin contents reveals lignin and flavonoid biosynthesis in Brassica napus

文献类型： 外文期刊

作者： Ding, Yiran 1 ; Yu, Shizhou 2 ; Wang, Jia 1 ; Li, Maoteng 3 ; Qu, Cunmin 1 ; Li, Jiana 1 ; Liu, Liezhao 1 ;

作者机构： 1.Southwest Univ, Acad Agr Sci, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang 550008, Guizhou, Peoples R China

3.Huazhong Univ Sci & Technol, Inst Resource Biol & Biotechnol, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China

关键词： Brassica napus L; Seed coat; Lignin; Flavonoid; RNA-Seq

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Brassica napus L. (2n = 38, AACC) is one of the most important oil crops and sources of protein for animal feed worldwide. Lignin is a large molecule aromatic polymer and a major cell wall component. However, lignin in the seed coat reduces the availability and restricts the development of rapeseed cake. Therefore, it is critical to reduce the lignin content of the seed coat. Here, high-lignin (H-lignin) and low-lignin (L-lignin) content recombinant inbred lines (RILs) were selected from an RIL population for analysis. Results The cross-section results indicated that the seed coat of the H-lignin lines was thicker than that of the L-lignin lines, especially the palisade layer. The seed coats and embryos at 35, 40 and 46 days after flowering (DAF) were subjected to RNA sequencing (RNA-Seq), and the expression of the BnPAL and BnC4H gene families in the lignin pathway was significantly higher in the H-lignin seed coat than in the L-lignin seed coat. The Bn4CL gene family also showed this trend. In addition, among the genes related to plant hormone synthesis, BnaC02g01710D was upregulated and BnaA07g11700D and BnaC09g00190D were downregulated in H-lignin lines. Some transcription factors were upregulated, such as BnNAC080, BnNAC083, BnMYB9, BnMYB9-1, BnMYB60 and BnMYB60-1, while BnMYB91 was downregulated in H-lignin lines. Moreover, most genes of the flavonoid pathway, such as BnCHS and BnDFR, were strongly expressed in H-lignin seed coat. Conclusions In Our study, some key genes such as hormone synthesis genes, transcription factors and miRNAs related to lignin and flavonoid biosynthesis were identified. A regulatory model of B. napus seed coat lignin was proposed. These results provide new insight into lignin and flavonoid biosynthesis in B. napus.

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