A RUBY Reporter for Efficient Banana Transformation and Development of Betalain-Rich Musa Germplasm
文献类型： 外文期刊
作者： He, Weidi 1 ; Huang, Huoqing 1 ; Wang, Shuxian 1 ; Wang, Dalin 1 ; Xie, Yanling 1 ; Hu, Chunhua 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res,Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Res Utili, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou 510640, Peoples R China
2.South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China
关键词： banana; betalain; genetic transformation; RUBY
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 16 期
页码：
收录情况： SCI
摘要： Bananas are economically important crops valued for both their nutritional and dietary uses. However, the global banana industry suffers from a narrow base dominated by a single variety. Developing novel varieties enriched in health-promoting compounds such as betalains can help diversify banana germplasm and meet evolving consumer demands. In this study, the RUBY reporter system was employed to produce betalain-rich bananas via stable and transient genetic transformations. Transient transformation by injecting 3 mL of Agrobacterium suspension into immature fruits produced vivid red-purple pulp containing up to 1.78 mg/g of betalains. For stable transformation, embryonic cell suspensions expressing RUBY exhibited a red-purple coloration after the first screening, reducing the selection period from 45 to 15 days. These findings demonstrate that RUBY is a reliable visual reporter for efficient screening and can be used to develop nutritionally enhanced bananas.
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