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Fusaric acid-mediated S-glutathionylation of MaAKT1 channel confers the virulence of Foc TR4 to banana

文献类型： 外文期刊

作者： Zhang, Jun 1 ; Liu, Siwen 3 ; Yang, Wenlong 3 ; Xie, Yanling 3 ; Shao, Chuange 3 ; Zhang, Zhi-Ren 1 ; Li, Chunyu 3 ; Yao, Xiaoqiang 2 ;

作者机构： 1.Harbin Med Univ, Dept Cardiol, NHC Key Lab Cell Transplantat, Affiliated Hosp 1,Key Labs Educ Minist Myocardial, Harbin, Peoples R China

2.Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Sch Biomed Sci, Hong Kong, Peoples R China

3.Guangdong Acad Agr Sci, Key Lab South Subtrop Fruit Biol & Genet Res Utili, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Inst Fruit Tree Res,Minist Agr & Rural Affairs, Guangzhou, Peoples R China

4.Guangdong Lab Lingnan Modern Agr, Guangzhou, Peoples R China

5.Guangdong Lab Lingnan Modern Agr, Maoming Branch, Maoming, Peoples R China

6.Chinese Univ Hong Kong, Ctr Cell & Dev Biol, Sch Life Sci, Hong Kong, Peoples R China

期刊名称：PLOS PATHOGENS （ 影响因子：4.9； 五年影响因子：5.4 ）

ISSN： 1553-7366

年卷期： 2025 年 21 卷 4 期

页码：

收录情况： SCI

摘要： Our previous studies have demonstrated that the phytotoxin fusaric acid (FSA), secreted by several Fusarium species, acts as a key factor in the development of plant diseases; however, the underlying mechanism remains unknown. In this study, we showed that the symptoms of Fusarium wilt in banana seedlings closely resembled those observed in plants grown under potassium (K+) deficiency conditions. Mechanistically, we found that FSA induces the accumulation of intracellular reactive oxygen species (ROS), which in turn inhibits banana K+ in banana roots. This inhibition occurs via S-glutathionylation of the banana AKT1 (MaAKT1) channel, leading to reduced K+ influx and reduced K+ content in banana roots. Through mutagenesis, electrophysiological studies, immunofluorescence staining, and co-immunoprecipitation experiment, we demonstrated that mutation of Cys202, a highly conserved site in the transmembrane segment 5 of MaAKT1, diminished the biochemical interaction of glutathione (GSH) and the channel induced by FSA, and alleviated Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) and FSA-induced yellowing symptom. The evolutionarily conserved function of this site for S-glutathionylation was also observed in Arabidopsis AKT1 (AtAKT1) channel, as mutation of its homologue site in AtAKT1 similarly reduced the GSH-AtAKT1 interaction under FSA stress. Collectively, our results suggest that FSA contributes to disease progression by decreasing K+ absorption through S-glutathionylation of MaAKT1 channel at the conserved Cys202 residue. These findings uncover a previously unrecognized role of FSA in regulating K+ homeostasis in bananas, and provide a foundation for future strategies to treat Fusarium wilt and increase banana production by targeting the conserved S-glutathionylation site in MaAKT1 channel.

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