文献类型： 外文期刊
作者： Xu, Hongyan 1 ; Ban, Wenzhuo 1 ; Tian, Jiaming 1 ; Xu, Jianfei 1 ; Tan, Zhimin 1 ; Li, Sendong 1 ; Chen, Kaili 1 ; Ou, Mi 3 ; Li, Kaibin 3 ;
作者机构： 1.Southwest Univ, Integrat Sci Ctr Germplasm Creat Western China CHO, Minist Educ, Chongqing 402460, Peoples R China
2.Southwest Univ, Coll Fisheries, Key Lab Freshwater Fish Reprod & Dev, Key Lab Aquat Sci Chongqing,Minist Educ, Chongqing 402460, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat &, Minist Agr, Guangzhou 510380, Peoples R China
关键词： Traf6-knockout; Liver dysfunctions; Defect of Meiotic germ cells in gonads; Zebrafish
期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.0； 五年影响因子：3.4 ）
ISSN： 1436-2228
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Traf6, an adaptor protein, exhibits non-conventional E3 ubiquitin ligase activity and was well studied as an important factor in immune systems and cancerogenesis. In mice, the traf6-null caused a perinatal death, so that the underlying pathophysiology of traf6-defeciency is still largely unclear in animals. Here, in the present study, a traf6 knockout zebrafish line (traf6(-/-)) was generated and could survive until adulthood, providing a unique opportunity to demonstrate the functions of traf6 gene in animals' organogenesis beyond the mouse model. The body of traf6(-/-) fish was found to be significantly shorter than that of the wildtype (WT). Likewise, a comparative transcriptome analysis showed that 866 transcripts were significantly altered in the traf6-/- liver, mainly involved in the immune system, metabolic pathways, and progesterone-mediated oocyte maturation. Especially, the mRNA expression of the pancreas duodenum homeobox protein 1 (pdx1), glucose-6-phosphatase (g6pcb), and the vitellogenesis genes (vtgs) were significantly decreased in the traf6(-/-) liver. Subsequently, the glucose was found to be accumulated in the traf6(-/-) liver tissues, and the meiotic germ cell was barely detected in traf6(-/-) testis or ovary. The findings of this study firstly implied the pivotal functions of traf6 gene in the liver and gonads' development in fish species.
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