Single-Cell Atlas of the Chinese Tongue Sole (Cynoglossus semilaevis) Ovary Reveals Transcriptional Programs of Oogenesis in Fish
文献类型： 外文期刊
作者： Liu, Xiang 1 ; Huang, Yingyi 1 ; Tan, Fujian 3 ; Wang, Hong-Yan 2 ; Chen, Jian-Yang 3 ; Zhang, Xianghui 2 ; Zhao, Xiaona 2 ; Liu, Kaiqiang 2 ; Wang, Qian 2 ; Liu, Shanshan 3 ; Piferrer, Francesc 9 ; Fan, Guangyi 3 ; Shao, Changwei 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China
3.BGI Shenzhen, BGI Qingdao, Qingdao, Peoples R China
4.BGI Shenzhen, Shenzhen, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
6.BGI Shenzhen, Qingdao Europe Adv Inst Life Sci, Qingdao, Peoples R China
7.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian, Peoples R China
8.Ningbo Univ, Sch Marine Sci, Ningbo, Peoples R China
9.CSIC, Inst Ciencies Mar ICM, Barcelona, Spain
关键词： scRNA-seq; oogenesis; Chinese tongue sole; germ cell; granulosa cell
期刊名称：FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY （ 影响因子：6.081； 五年影响因子：6.576 ）
ISSN： 2296-634X
年卷期： 2022 年 10 卷
页码：
收录情况： SCI
摘要： Oogenesis is a highly orchestrated process that depends on regulation by autocrine/paracrine hormones and growth factors. However, many details of the molecular mechanisms that regulate fish oogenesis remain elusive. Here, we performed a single-cell RNA sequencing (scRNA-seq) analysis of the molecular signatures of distinct ovarian cell categories in adult Chinese tongue sole (Cynoglossus semilaevis). We characterized the successive stepwise development of three germ cell subtypes. Notably, we identified the cellular composition of fish follicle walls, including four granulosa cell types and one theca cell type, and we proposed important transcription factors (TFs) showing high activity in the regulation of cell identity. Moreover, we found that the extensive niche-germline bidirectional communications regulate fish oogenesis, whereas ovulation in fish is accompanied by the coordination of simultaneous and tightly sequential processes across different granulosa cells. Additionally, a systems biology analysis of the homologous genes shared by Chinese tongue sole and macaques revealed remarkably conserved biological processes in germ cells and granulosa cells across vertebrates. Our results provide key insights into the cell-type-specific mechanisms underlying fish oogenesis at a single-cell resolution, which offers important clues for exploring fish breeding mechanisms and the evolution of vertebrate reproductive systems.
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