Seed specifically over-expressing DGAT2A enhances oil and linoleic acid contents in soybean seeds
文献类型： 外文期刊
作者： Jing, Guangqin 1 ; Tang, Daoping 1 ; Yao, Yao 2 ; Su, Youke 1 ; Shen, Yue 3 ; Bai, Yang 1 ; Jing, Wen 1 ; Zhang, Qun 1 ; Lin 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
2.Jilin Acad Agr Sci, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China
关键词： Active sites; GmDGAT2A; N-terminus; Oil; Soybean
期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）
ISSN： 0006-291X
年卷期： 2021 年 568 卷
页码：
收录情况： SCI
摘要： Triacylglycerol (TAG), a main component of oil, is mainly biosynthesized by diacylglycerol acyltransferase (DGAT), which is critical for oil accumulation in plants. Intensive focus has been on DGAT2 functioning in unsaturated fatty acids biosynthesis. In this study, we analyzed the coding sequence (CDS) and amino acid sequence of GmDGAT2A and determined its key active sites through site-directed mutagenesis. As a consequence, H132, G201, and P152-X-I154-K155 were found to be essential active sites for GmDGAT2A. The spatial structure of the protein may bring the three active sites into close proximity, constituting an active domain. Additionally, N-terminus of GmDGAT2A was found to be an important regulator for the activity. Further, in vitro activity results uncovered GmDGAT2A was prone to utilize C18:2-CoA as the substrate. Consequently, overexpression of GmDGAT2A driven by a seed-specific promoter of Gmole1 in soybean significantly increased linoleic acid content specifically and total oil content, concomitant with accelerated elongation. (c) 2021 Published by Elsevier Inc.
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