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Sustainable valorizing high-protein feather waste utilization through solid-state fermentation by keratinase-enhanced Streptomyces sp. SCUT-3 using a novel promoter

文献类型： 外文期刊

作者： Lu, Wen-Jun 1 ; Zhang, Ming-Shu 1 ; Lu, De-Lin 1 ; Li, Zhi-Wei 1 ; Yang, Zhen-Dong 1 ; Wu, Lei 1 ; Ni, Jing-Tao 1 ; Chen, Wei-Dong 3 ; Deng, Jun-Jin 1 ; Luo, Xiao-Chun 1 ;

作者机构： 1.South China Univ Technol, Guangzhou Higher Educ Mega Ctr, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, State Key Lab Swine & Poultry Breeding Ind, Guangdong Key Lab Crop Germplasm Resources Conserv, Guangzhou 510640, Peoples R China

3.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China

关键词： Feather waste; Sustainable valorization; Promoter optimization; Keratinase enhancement; Feed protein resource

期刊名称：WASTE MANAGEMENT （ 影响因子：8.1； 五年影响因子：8.7 ）

ISSN： 0956-053X

年卷期： 2024 年 174 卷

页码：

收录情况： SCI

摘要： Feather waste, a rich source of proteins, has traditionally been processed through high-temperature puffing and acid-base hydrolysis, contributing to generation of greenhouse gases and H2S. To address this issue, we employed circular economy techniques to recover the nutritional value of feather waste. Streptomyces sp. SCUT-3, an efficient proteolytic and chitinolytic bacterium, was isolated for feather degradation previously. This study aimed to valorize feather waste for feed purposes by enhancing its feather transformation ability through promoter optimization. Seven promoters were identified through omics analysis and compared to a common Streptomyces promoter ermE*p. The strongest promoter, p24880, effectively enhanced the expression of three candidate ker-atinases (Sep39, Sep40, and Sep53). The expression efficiency of double-, triple-p24880 and sandwich p24880- sep39-p24880 promoters were further verified. The co-overexpression strain SCUT-3-p24880-sep39-p24880-sep40 exhibited a 16.21-fold increase in keratinase activity compared to the wild-type. Using this strain, a solid-state fermentation process was established that increased the feather/water ratio (w/w) to 1:1.5, shortened the fermentation time to 2.5 days, and increased soluble peptide and free amino acid yields to 0.41 g/g and 0.14 g/g, respectively. The resulting has high protein content (90.49 %), with high in vitro digestibility (94.20 %). This method has the potential to revolutionize the feather waste processing industry.

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