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The Anti-Inflammatory Properties of Polysaccharides Extracted from Moringa oleifera Leaves on IEC6 Cells Stimulated with Lipopolysaccharide In Vitro

文献类型： 外文期刊

作者： Husien, Hosameldeen Mohamed 1 ; Peng, Weilong 1 ; Essa, Mohamed Osman Abdalrahem 1 ; Adam, Saber Y. 4 ; Ur Rehman, Shahab 3 ; Ali, Rahmat 3 ; Saleh, Ahmed A. 4 ; Wang, Mengzhi 3 ; Li, Jingui 1 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China

2.Albutana Univ, Coll Vet Med, Rufaa 22217, Sudan

3.Yangzhou Univ, Coll Anim Sci & Technol, Lab Metab Manipulat Herbivorous Anim Nutr, Yangzhou 225009, Peoples R China

4.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China

5.Alexandria Univ, Fac Agr Al Shatby, Anim & Fish Prod Dept, Alexandria 11865, Egypt

6.Xinjiang Acad Agr Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Peoples R China

关键词： M. oleifera; intestinal epithelial cells; signalling pathways; inflammatory response; gut barrier

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 23 期

页码：

收录情况： SCI

摘要： Moringa oleifera (M. oleifera) is a plant with significant medicinal and nutritional value and contains various bioactive compounds, particularly in its leaves (MOL). This study sought to explore the impact of M. oleifera leaf polysaccharides (MOLPs) on lipopolysaccharide (LPS)-activated intestinal epithelial cells (IEC6) and to uncover the mechanisms involved. The cytotoxicity of MOLP on IEC6 cells was assessed using the Cell Counting Kit-8 (CCK-8) assay, which demonstrated a safe concentration range of 0-1280 mu g/mL. The impact of MOLP on cell viability was further evaluated over 12 to 48 h. IEC6 cells were treated with three concentrations of MOLP low (25 mu g/mL), medium (50 mu g/mL), and high (100 mu g/mL) alongside LPS (50 mu g/mL) stimulation for one day. The findings revealed that treatment with MOLP significantly promoted cell migration and increased the production of interleukin-10 (IL-10), while it simultaneously decreased cell apoptosis and the levels of pro-inflammatory cytokines, such as tumour necrosis factor alpha (TNF-alpha), interleukin 1 beta (IL-1 beta), and interleukin 6 (IL-6). Additionally, MOLP treatments across all concentrations significantly reduced the expression of Toll-like receptor 4 (TLR-4), myeloid differentiation primary response 88 (MyD88), phosphorylated nuclear factor kappa B-alpha (pI kappa B-alpha), and phosphorylated NF-kappa B p65 signalling pathways. Moreover, MOLP restored the expression of tight junction proteins, such as zonula occludens-1 (ZO-1) and occludin, which had been disrupted by LPS. These results indicate that MOLP exhibits anti-inflammatory properties by inhibiting inflammatory signalling pathways and maintaining intestinal barrier integrity through the upregulation of tight junction proteins in IEC6 cells. This study enhances our understanding of the anti-inflammatory capabilities of MOLP.

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