Determination of chloramphenicol residues in foods by ELISA and LC-MS/MS coupled with molecularly imprinted solid phase extraction
文献类型： 外文期刊
作者： Shi, Xizhi 1 ; Song, Suquan 2 ; Sun, Aili 1 ; Li, Dexiang 1 ; Wu, Aibo 2 ; Zhang, Dabing 2 ;
作者机构： 1.Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China
2.Shanghai Jiao Tong Univ, Bor Luh Food Safety Ctr, Coll Life Sci & Biotechnol, Shanghai 200030, Peoples R China
3.Shanghai Acad Agr Sci, Inst Agri Food Stand & Testing Technol, Shanghai, Peoples R China
关键词： Chloramphenicol;Foods;Molecularly imprinted polymer microspheres
期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）
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收录情况： SCI
摘要： The combination of molecularly imprinted solid-phase extraction (MISPE) with ELISA and LC-MS/MS was developed for the detection of chloramphenicol (CAP) in honey samples. Significant recoveries of 99.1±7.1 and 98.8±8.2% were obtained for intraand inter-assay determination by ELISA determination, respectively. The limit of detection of CAP was 0.034 μgkg~(-1) and the limit of quantification was 0.046 μgkg~(-1). Determination and validation of CAP by using LC-MS/MS were performed following the same extraction and purification process as for the ELISA. The results demonstrated that the CAP samples purified by using MISPE were simultaneously applicable to analysis by ELISA and LC-MS/MS.
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