您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces)

文献类型： 外文期刊

作者： Li, Ping 1 ; Hulak, Martin 1 ; Rodina, Marek 1 ; Sulc, Miroslav; Li, Zhi-Hua 1 ; Linhart, Otomar 1 ;

作者机构： 1.Univ S Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, S Bohemian Res Ctr Aquaculture & Biodivers Hydroc, Vodnany 38925, Czech Republic

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodivers Conservat & Utilizat, Jinzhou, Hubei, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodivers Conservat & Utilizat, Jinzhou, H

关键词： Endangered species;Sperm;Comparative proteomics;MALDI-TOF/MS

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.674； 五年影响因子：2.941 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Sturgeon and paddlefish (Acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: Siberian sturgeon (Acipenser baerii), sterlet (A. ruthenus), Russian sturgeon (A. gueldenstaedtii), starry sturgeon (A. stellatus), beluga (Huso huso), and Mississippi paddlefish (Polyodon spathula). Protein profiles of spermatozoa were determined by isoelectric focusing and two-dimensional electrophoresis (2-DE) high-resolution gels. The peptides, previously selected by 2-DE analysis as potentially species-specific, were obtained by "in-gel" tryptic digestion, followed by matrix-associated laser desorption/ionization time-of-flight/mass spectrometry (MALDI-TOF/MS). Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. Exceptions were A. ruthenus and H. huso, species with a close phylogenetic relationship. Glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula. Phosphoglycerate kinase was detected only in A. ruthenus and H. huso, and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso. This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level.

  • 相关文献

[1]Complete mitochondrial genome of catfish Bagarius bagarius (Hamilton, Sisoridae; Siluriformes) from Indus River Sindh, Pakistan. Lashari, Punhal,Deng, Yulin,Lashari, Punhal,Laghari, Muhammad Younis,Xu, Peng,Zhao, Zixia,Jiang, Li,Sun, Xiaowen,Zhang, Yan,Lashari, Punhal,Laghari, Muhammad Younis,Narejo, Naeem Tariq.

[2]Complete mitochondrial genome of the Freshwater Catfish Rita rita (Siluriformes, Bagridae). Lashari, Punhal,Deng, Yulin,Lashari, Punhal,Laghari, Muhammad Younis,Xu, Peng,Zhao, Zixia,Jiang, Li,Sun, Xiaowen,Zhang, Yan,Lashari, Punhal,Laghari, Muhammad Younis,Narejo, Naeem Tariq. 2015

[3]Genetic diversity of the endangered scaly-sided merganser (Mergus squamatus) in the wintering habitat of central-southern China. Zhang, Z-Q,Wang, A-P,Luo, C-H,Qin, C-J,Fang, D-A,Liu, K.. 2013

[4]Complete mitochondrial genome of burbot (Lota lota: family Gadidae) from Amur basin lineage, Northeastern China. Lashari, Punhal,Laghari, Muhammad Younis,Xu, Peng,Zhao, Zi-Xia,Jiang, Li,Sun, Xiao-Wen,Zhang, Yan. 2016

[5]Isolation and characterization of eleven novel microsatellite loci of Brachymystax lenok tsinlingensis, a threatened fish endemic to Shaanxi, China. Liu, Haixia,Li, Yang,Liu, Xiaolin,Zou, Guiwei,Wei, Qiwei,Zou, Guiwei,Wei, Qiwei,Zou, Guiwei,Wei, Qiwei. 2013

[6]Development of 27 novel cross-species microsatellite markers for the endangered Hucho bleekeri using next-generation sequencing technology. Wang, Ke,Wei, Qiwei,Zhang, Shuhuan,Wang, Dengqiang,Xin, Miaomiao,Wu, Jinming,Sun, Qingliang,Du, Hao,Wang, Chengyou,Huang, Jun,Wei, Qiwei.

[7]Sperm of the giant grouper: cryopreservation, physiological and morphological analysis and application in hybridizations with red-spotted grouper. Tian, Yongsheng,Jiang, Jing,Wang, Na,Qi, Wenshan,Liang, You,Chen, Songlin,Jiang, Jing,Qi, Wenshan,Yang, Chuanjun,Zhai, Jieming,Li, Bo,Chen, Youming.

[8]Sperm cryopreservation of sex-reversed seven-band grouper, Epinephelus septemfasciatus. Tian, Yongsheng,Qi, Wenshan,Jiang, Jing,Wang, Na,Wang, Di,Chen, Chao,Chen, Songlin,Qi, Wenshan,Jiang, Jing,Wang, Di,Zhai, Jieming.

[9]Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae). Liu, XZ,Zhang, SC,Zhang, YZ,Xu, YJ. 2006

[10]Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application. Ding, Fuhong,Lei, Jilin,Lall, Santosh P.,Milley, Joyce E.,Li, Jun,Rommens, Melissa. 2011

[11]Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization. Ji, XS,Chen, SL,Tian, YS,Yu, GC,Sha, ZX,Xu, MY,Zhang, SC. 2004

[12]Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques. Li, Ping,Li, Zhi-Hua,Dzyuba, Boris,Hulak, Martin,Rodina, Marek,Linhart, Otomar,Li, Ping,Li, Zhi-Hua,Dzyuba, Boris. 2010

[13]Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization. Chen, SL,Ji, XS,Yu, GC,Tian, YS,Sha, ZX.

[14]Cryopreservation of common carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues. Li, P.,Hulak, M.,Li, Z. H.,Psenicka, M.,Rodina, M.,Gela, D.,Linhart, O.,Li, P.,Li, Z. H.,Sulc, M..

[15]Sperm nuclear transfer and transgenic production in the fish medaka. Liu, Tongming,Hong, Yunhan,Liu, Ling,Wei, Qiwei,Hong, Yunhan.

[16]Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology. Zhang, YZ,Zhang, SC,Liu, XZ,Xu, YY,Wang, CL,Sawant, MS,Li, J,Chen, SL.

[17]The protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish (Takifugu obscurus) under low temperature stress. Cheng, Chang-Hong,Liang, Hai-Yan,Luo, Sheng-Wei,Wang, An-Li,Ye, Chao-Xia. 2018

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台