Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)
文献类型： 外文期刊
作者： Ji, Xiang-Shan 1 ; Chen, Song-Lin 1 ; Yang, Jing-Feng 1 ; Ma, Hong-Yu 1 ; Jiang, Yun-Liang 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Shandong Agr Univ, Coll Anim Sci & Technol, Tai An 271018, Shandong, Peoples R China
关键词： sex ratio;diploid gynogenetic
期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.235； 五年影响因子：2.616 ）
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收录情况： SCI
摘要： In the present study, methodology of gynogenetic induction in spotted halibut were developed and optimized; the sex ratio of putative meiotic gynogenetic diploids was determined using AFLP-based molecular sexing technique; the homozygosity of gynogenetic population was assessed as opposed to cultivated population. The results showed that high percentage of meiotic gynogenetic diploids were generated when the eggs fertilized with irradiated heterologous sea perch frozen sperm (30-50 mJ cm(-2)) were cold shocked in sea water of -1A degrees C for 40-75 min at 5 min after fertilization. About 15,200 diploid gynogenetic larvae were achieved and they exhibited normal morphology similar to diploid control. The gynogenetic diploids were 100% female, which first confirmed the female homogamete (XX/XY) sex determination in spotted halibut. The genetic analysis showed that the average H (O) was, respectively, 0.404 and 0.724 in gynogenetic population and cultivated population, indicating an increase of homozygosity in gynogenetic population.
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