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High Sensitive Detection of Cry1Ab Protein Using a Quantum Dot-Based Fluorescence-Linked Immunosorbent Assay

文献类型： 外文期刊

作者： Zhu, Xiaolei 1 ; Chen, Lili 2 ; Shen, Ping 3 ; Jia, Junwei 4 ; Zhang, Dabing 1 ; Yang, Litao 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Natl Mol Characterizat Ctr Genetically Modified O, Shanghai 200240, Peoples R China

2.Shanghai Normal Univ, Coll Life & Environm Sci, Lab Plant Biotechnol, Shanghai 200234, Peoples R China

3.Minist Agr, Dev Ctr Sci & Technol, Beijing 100026, Peoples R China

4.Shanghai Acad Agr Sci, Agro Biotech Res Ctr, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

5.Shanghai Jiao Tong Univ, Minist Educ, Key Lab Genet & Dev & Neuropsychiat Dis, Bio X Res Ctr, Shanghai 200240, Peoples R China

关键词： Cry1Ab;genetically modified maize;quantum dot;QD-FLISA

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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年卷期：

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收录情况： SCI

摘要： Protein-based detection methods, enzyme-linked immunosorbent assay (ELISA) and lateral flow strip, have been widely used for rapid, spot, and sensitive detection of genetically modified organisms (GMOs). Herein, one novel quantum dot-based fluorescence-linked immunosorbent assay (QD-FLISA) was developed employing quantum dots (QDs) as the fluorescent marker for the detection of the Cry1Ab protein in MON810 maize. The end-point fluorescent detection system was carried out using QDs conjugated with goat anti-rabbit secondary antibody. The newly developed Cry1Ab QD-FLISA assay was highly specific to the Cry1Ab protein and had no cross-reactivity with other target proteins, such as Cry2Ab, Cry1F, and Cry3Bb. The quantified linearity was. achieved in the value range of 0.05—5% (w/w). The limits of detection (LOD) and quantification (LOQ) of the QD-FLISA were 2.956 and 9.854 pg/mL, respectively, which were more sensitive than the conventional sandwich ELISA method. All of the results indicated that QD-FLISA was a highly specific and sensitive method for the monitoring of Cry1Ab in GMOs.

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