Enhancement of artemisinin content in tetraploid Artemisia annua plants by modulating the expression of genes in artemisinin biosynthetic pathway
文献类型: 外文期刊
作者: Lin, Xiuyan 1 ; Zhou, Yin; Zhang, Jianjun; Lu, Xu 1 ; Zhang, Fangyuan 1 ; Shen, Qian 1 ; Wu, Shaoyan 1 ; Chen, Yunf 1 ;
作者机构: 1.Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Plant Biotechnol Res Ctr, Key Lab Urban Agr S,Minist Agr,Sch Agr & Biol, Shanghai 200240, Peoples R China
2.Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Plant Biotechnol Res Ctr, Key Lab Urban Agr S,Minis
关键词: Artemisia annua L.;Colchicine;Flow cytometry;Gene expression;Stomata;Tetraploid
期刊名称:BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY ( 影响因子:2.431; 五年影响因子:2.124 )
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收录情况: SCI
摘要: Tetraploid Artemisia annua plants were successfully inducted by using colchicine, and their ploidy was confirmed by flow cytometry. Higher stomatal length but lower frequency in tetraploids were revealed and could be considered as indicators of polyploidy. The average level of artemisinin in tetraploids was increased from 39% to 56% than that of the diploids during vegetation period, as detected by high-performance liquid chromatography-evaporative light scattering detector. Gene expressions of 10 key enzymes related to artemisinin biosynthetic pathway in different ploidy level were analyzed by semiquantitative polymerase chain reaction and significant upregulation of FPS, HMGR, and artemisinin metabolite-specific Aldh1 genes were revealed in tetraploids. Slight increased expression of ADS was also detected. Our results suggest that higher artemisinin content in tetraploid A. annua may result from the upregulated expression of some key enzyme genes related to artemisinin biosynthetic pathway.
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