Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal
文献类型： 外文期刊
作者： Wang, Hong-Yan 1 ; Liu, Xiang 1 ; Chen, Jian-Yang 4 ; Huang, Yingyi 1 ; Lu, Yifang 1 ; Tan, Fujian 4 ; Liu, Qun 4 ; Yang, Mingming 4 ; Li, Shuo 1 ; Zhang, Xianghui 1 ; Qin, Yating 4 ; Ma, Wenxiu 1 ; Yang, Yingming 1 ; Meng, Liang 4 ; Liu, Kaiqiang 1 ; Wang, Qian 1 ; Fan, Guangyi 4 ; Nobrega, Rafael H. 10 ; Liu, Shanshan 4 ; Piferrer, Francesc 11 ; Shao, Changwei 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao 266072, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.BGI, BGI Res Qingdao, Qingdao 266555, Peoples R China
5.BGI Shenzhen, Shenzhen 518083, Peoples R China
6.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China
7.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian 116023, Peoples R China
8.BGI Shenzhen, Qingdao Europe Adv Inst Life Sci, Qingdao 266555, Peoples R China
9.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China
10.Sao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, Reprod & Mol Biol Grp,UNESP, Botucatu, SP, Brazil
11.CSIC, Inst Ciencies Mar ICM, Barcelona, Spain
关键词： Cynoglossus semilaevis; spermatogenesis; single-cell transcriptome; sex reversal; spermatogonia
期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：10.372； 五年影响因子：7.587 ）
ISSN： 1674-7305
年卷期：
页码：
收录情况： SCI
摘要： Female-to-male sex reversals (pseudomales) are common in lower vertebrates and have been found in natural populations, which is a concern under rapid changes in environmental conditions. Pseudomales can exhibit altered spermatogenesis. However, the regulatory mechanisms underlying pseudomale spermatogenesis remain unclear. Here, we characterized spermatogenesis in Chinese tongue sole (Cynoglossus semilaevis), a species with genetic and environmental sex determination, based on a high-resolution single-cell RNA-seq atlas of cells derived from the testes of genotypic males and pseudomales. We identified five germ cell types and six somatic cell types and obtained a single-cell atlas of dynamic changes in gene expression during spermatogenesis in Chinese tongue sole, including alterations in pseudomales. We detected decreased levels of Ca2+ signaling pathway-related genes in spermatogonia, insufficient meiotic initiation in spermatocytes, and a malfunction of somatic niche cells in pseudomales. However, a cluster of CaSR genes and MAPK signaling factors were upregulated in undifferentiated spermatogonia of pseudomales. Additionally, we revealed that Z chromosome-specific genes, such as piwil2, dhx37, and ehmt1, were important for spermatogenesis. These results improve our understanding of reproduction after female-to-male sex-reversal and provide new insights into the adaptability of reproductive strategies in lower vertebrates.
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