文献类型： 外文期刊

作者： Yuan, Xuejun ¹ ; Wang, Zhiyong ¹ ; Liu, Jianxiu ¹ ; She, Jianming ³ ;

作者机构： 1.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Grass Res Ctr, Nanjing 210014, Peoples R China

2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Biotechnol, Nanjing 210014, Peoples R China

关键词： Eremochloa ophiuroides;MH - lawns and turf;MH - turf grasses;MH - micropropagation;MH - callus;MH - seeds;MH - explants;MH - culture media;MH - mannitol;MH - coconut milk;MH - benzyladenine;MH - naphthaleneacetic acid;MH - rooting;MH - shoots;MH - in vitro regeneration

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

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收录情况： SCI

摘要： The objective of this study is to establish plant regeneration system with the seed of the new Chinese selection E-126of centipedegrass [Eremochloa ophiuroides (Munro.) Hack] as explant. In present study, the following results were obtained: (1) The medium formulation most suitable for calluses induction was identified to be MS with 1.0mglp# 2,4-D+30glp# mannitol+50mllp# coconut milk and the ratio of calluses induction was 96.0%, including 5.2% of yellow granule calluses induction. The above medium formulation was adopted for subculture. (2) The rate of shoot regeneration from yellow granular calluses was 98.0% by MS optimum medium formulation with 2.0mglp# KT+50mllp# coconut milk. The differentiated rate retained as high as 88.0% even after 5 times of subculture and 18.6% after 15 times of subculture. The optimum medium formulation for shoot growth was identified to be MS medium plus 2.0mglp# BAP, 0.8mglp# NAA and 50mllp# coconut milk. (3) The optimum medium for shoot rooting was identified to be MS medium with 0.6mglp# NAA+50mllp# coconut milk, and the rooting rate to be 98.0%. The survival rate of transplanted plantlets from tubes to basin with soil was 92.0%. In conclusion, the plant regeneration system was successfully developed in this study, which may provide basic reference for screening of somaclonal variants and genetic transformation of centipedegrass.