文献类型： 外文期刊

作者： Zong, Junqin ¹ ; Liu, Jianxiu ¹ ; Huang, Junqi ² ;

作者机构： 1.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing Bot Garden, Nanjing, Peoples R China

2.Jiangsu Acad Agr Sci, Nanjing, Jiangsu, Peoples R China

关键词： tall fescue;bar gene;pollen tube pathway;biology identification;PCR detection

期刊名称：PROCEEDINGS OF THE IIND INTERNATIONAL CONFERENCE ON TURFGRASS SCIENCE AND MANAGEMENT FOR SPORTS FIELDS

ISSN： 0567-7572

年卷期： 2008 年 783 期

页码：

收录情况： SCI

摘要： Frequent uses of herbicides in turfgrass management causes not only phytotoxicity to turfgrass but also environmental pollution. Genetic transformation of herbicide resistance gene into turfgrasses could prevent herbicide injury to non-target plants such as tall fescue. In this studu, Bar gene was transformed into two well performed selections of Festuca arundinacea cv. Houndog 5 via pollen-tube pathway method. Eighteen plants were alive after screening the Basta (4-(hydroxyl (methyl)phosphinoyl)-DL-homoalanine) resistant from 1286 plants; six plants (i.e., 572-1, 572-4,578-1, 578-3, 578-5 and 578-9) among eighteen plants have the DNA fragment with the same size as positive control via PCR detection. The transformation rate was 0.47%. The results suggest that the pollen tube pathway method could be feasibly used in the gene transformation for tall fescue.