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A protein coding for a pollen-specific gene in alfalfa (Medicago sativa L.) is localized mainly in the intine of the pollen wall

文献类型： 外文期刊

作者： Agyare-Tabbi, Akwasi 1 ; Zhang, Jian 1 ; Xiong, Ai-Sheng 4 ; Atnaseo, Chuthamat 1 ; Bahramnejad, Bahman 1 ; Erickso 1 ;

作者机构： 1.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada

2.Alberta Innovates Technol Futures Alberta Res Cou, Vegreville, AB T9C 1T4, Canada

3.Univ Kurdistan, Dept Agron & Plant Breeding, Sanandaj, Iran

4.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

关键词： cDNA: complementary DNA;arabinogalactan: 9036-66-2;PO2 protein: expression;genome

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

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年卷期：

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收录情况： SCI

摘要： In previous work, we characterized several cDNA and genomic clones of genes expressed in the pollen of alfalfa (Medicago sativa L.), also known as lucerne. In this study, we have extended that work and focused on expression at the protein level of one of those genes, PO2, a low-copy gene of unknown function, but with similarities to arabinogalactan proteins. Polyclonal antibodies, specific to the PO2 protein expressed in Escherichia coli, were raised in chickens, extracted from the eggs, and used in Western blots and immunolocalization studies to identify the location of the PO2 protein in sections of mature alfalfa pollen grains and pollen tubes. Our Western blot and immunolocalization data suggest that the PO2 protein is secreted, occurs largely in the intine, and is not detectable in the pollen tube; moreover, it is not detectable on the surface of the pollen grain, nor does it diffuse out of the pollen grain into an aqueous medium.

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