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Phytoremediation of triphenylmethane dyes by overexpressing a Citrobacter sp triphenylmethane reductase in transgenic Arabidopsis

文献类型: 外文期刊

作者: Fu, Xiao-Yan 1 ; Zhao, Wei 1 ; Xiong, Ai-Sheng 1 ; Tian, Yong-Sheng 1 ; Zhu, Bo 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;

作者机构: 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Biotechnol Res Inst, Shanghai, Peoples R China

关键词: Phytoremediation;Citrobacter sp.;Triphenylmethane reductase;Triphenylmethane dyes;Transgenic Arabidopsis

期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )

ISSN: 0175-7598

年卷期: 2013 年 97 卷 4 期

页码:

收录情况: SCI

摘要: Triphenylmethane dyes are extensively utilized in textile industries, medicinal products, biological stains, and food processing industries, etc. They are generally considered as xenobiotic compounds, which are very recalcitrant to biodegradation. The widespread persistence of such compounds has generated concerns with regard to remediation of them because of their potential carcinogenicity, teratogenicity, and mutagenicity. In this study, we present a system of phytoremediation by Arabidopsis plants developed on the basis of overexpression of triphenylmethane reductase (TMR) from the Citrobacter sp. The morphology and growth of TMR transgenic Arabidopsis plants showed significantly enhanced tolerances to crystal violet (CV) and malachite green (MG). Further, HPLC and HPLC-MS analyses of samples before and after dye decolorization in culture media revealed that TMR transgenic plants exhibited strikingly higher capabilities of removing CV from their media and high efficiencies of converting CV to non-toxic leucocrystal violet (LCV). This work indicates that microbial degradative gene may be transgenically exploited in plants for bioremediation of triphenylmethane dyes in the environment.

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