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Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation

文献类型： 外文期刊

作者： Zhang, Wen-Hui 1 ; Deng, Yong-Dong 1 ; Chen, Zhi-Feng 3 ; Zuo, Zhi-Hao 1 ; Tian, Yong-Sheng 1 ; Xu, Jing 1 ; Wang, Bo 1 ; Wang, Li-Juan 1 ; Han, Hong-Juan 1 ; Li, Zhen-Jun 1 ; Wang, Yu 1 ; Yao, Quan-Hong 1 ; Gao, Jian-Jie 1 ; Fu, Xiao-Yan 1 ; Peng, Ri-He 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Safety Assessment Environm Agr Genet Modif, Beijing, Peoples R China

3.Zunyi Normal Coll, Coll Biol & Agr Technol, Zunyi, Peoples R China

关键词： 4-Dinitrotoluene; Re-synthesize gene; Complete degradation; Bioremediation; Escherichia coli

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 262 卷

页码：

收录情况： SCI

摘要： 2,4-Dinitrotoluene (2,4-DNT) as a common industrial waste has been massively discharged into the environment with industrial wastewater. Due to its refractory degradation, high toxicity, and bioaccumulation, 2,4-DNT pollution has become increasingly serious. Compared with the currently available physical and chemical methods, in situ bioremediation is considered as an economical and environmentally friendly approach to remove toxic compounds from contaminated environment. In this study, we relocated a complete degradation pathway of 2,4-DNT into Escherichia coli to degrade 2,4-DNT completely. Eight genes from Burkholderia sp. strain were re-synthesized by PCR-based two-step DNA synthesis method and introduced into E. coli. Degradation experiments revealed that the transformant was able to degrade 2,4-DNT completely in 12 h when the 2,4-DNT concentration reached 3 mM. The organic acids in the tricarboxylic acid cycle were detected to prove the degradation of 2,4-DNT through the artificial degradation pathway. The results proved that 2,4-DNT could be completely degraded by the engineered bacteria. In this study, the complete degradation pathway of 2,4-DNT was constructed in E. coli for the first time using synthetic biology techniques. This research provides theoretical and experimental bases for the actual treatment of 2,4-DNT, and lays a technical foundation for the bioremediation of organic pollutants.

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