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Development of "environmentally friendly" super Escherichia coli strains that can completely biodegrade toluene

文献类型： 外文期刊

作者： Wang, Yu 1 ; Qian, Cen 1 ; Deng, Yong-Dong 2 ; Tian, Yong-Sheng 1 ; Gao, Jian-Jie 1 ; Xu, Jing 1 ; Li, Zhen-Jun 1 ; Fu, Xiao-Yan 1 ; Sun, Yuan-Zhang 3 ; Han, Hong-Juan 1 ; Zhang, Wen-Hui 1 ; Zhang, Hao 1 ; Wang, Bo 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Safety Assessment Environm Agr Genet Modif, Shanghai, Peoples R China

3.Qingdao Vland Saide Biotechnol Co Ltd, Shanghai, Peoples R China

关键词： Toluene; Biodegradation; Environmental pollution; Engineered bacteria; Synthetic biology

期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：13.2； 五年影响因子：13.5 ）

ISSN： 1385-8947

年卷期： 2025 年 506 卷

页码：

收录情况： SCI

摘要： The uncontrolled spread and accumulation of toxic toluene in soil and water present significant challenges in containment and management. Microorganisms have natural advantages in remediating pollutants, but the scarcity of efficient, complete degradation and friendly bacteria hinders their application in toluene remediation. In response to these challenges, this study focuses on the development and evaluation of engineered strain scavengers for toluene bioremediation. We redesigned and assembled a new pathway containing 11 genes for complete degradation of toluene and validated it in E. coli. Briefly, the modified strain can rapidly degrade 3 mM toluene within 3 h. Also, scanning electron microscopy showed that the engineered strain is more tolerant to 2 mM toluene than the control strain. By using the isotope tracing method, the metabolites of 13C-toluene were found incorporated into the tricarboxylic acid cycle in the engineering strain within 24 h. The most significant finding of this research is that these engineered strains can rapidly and completely remedy toluene-contaminated soil (294 mg Kg-1) under simulated conditions without adversely impacting soil microbial communities. Further, zebrafish embryos grew and developed normally in the toluene-contaminated water that had been repaired for 5 h, verifying the powerful detoxification and decontamination capabilities of the engineered strain. This "super" engineered bacterial scavenger, developed using synthetic biology technology, shows great potential and offers feasible strategies for the bioremediation of toluene contaminated sites.

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