文献类型： 外文期刊
作者： Wang, Bo 1 ; Wang, Yahui 1 ; Deng, Yuanjie 1 ; Yao, Quanhong 2 ; Xiong, Aisheng 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Biotechnol Res Inst, Shanghai 201106, Peoples R China
关键词： Daucus carota L.; Betanin; Biosynthesis; Metabolic engineering; Bioforti fication
期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：5.4 ）
ISSN： 2095-9885
年卷期： 2024 年 10 卷 3 期
页码：
收录情况： SCI
摘要： Betalain has received increased attention because of its high nutritional value and crucial physiological functions. Based on the elucidation of its core biosynthetic pathway, betalain can be produced in additional plants by metabolic engineering. Synthesis of betalain in carrot (Daucus carota L.) can improve its nutritional quality and economic value by extracting betalain from the fleshy root, non-edible part, and processing residue of carrot. In this study, two different constructs, namely, pYB:mCD (AomelOS, BvCYP76AD1S, and BvDODA1S) and pYB:CDD (BvCYP76AD1S, BvDODA1S, and MjcDOPA5GTS), were introduced into carrot for betanin synthesis by Agrobacterium-mediated transformation. Betanin can be synthetized in both transgenic calli, and pYB:mCD-transgenic callus can be used to produce betacyanin by suspension culture. However, pYB:mCD-transgenic seedlings can synthetize betanin only by tyrosine feeding. The pYB:CDD-transgenic lines can synthetize betanin in whole plants. The betanin content in fleshy root of pYB:CDD-transgenic carrot was (63.4 +/- 9) mg $g-1 fresh weight according to quantitative analysis. These betanin-producing carrot plant materials can be used to synthesize betanin for industrial application or consumption as dietary sources.
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