您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Ectopic expression of an Old Yellow Enzyme (OYE3) gene from Saccharomyces cerevisiae increases the tolerance and phytoremediation of 2-nitroaniline in rice

文献类型： 外文期刊

作者： Li, Zhenjun 1 ; Gao, Jianjie 1 ; Wang, Bo 1 ; Zhang, Hao 1 ; Tian, Yongsheng 1 ; Peng, Rihe 1 ; Yao, Quanhong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China

关键词： 2-nitroaniline; Old Yellow Enzyme; Phytoremediation; Reactive oxygen species; Saccharomyces cerevisiae

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2024 年 906 卷

页码：

收录情况： SCI

摘要： 2-nitroaniline (2 -NA) is an environmental pollutant and has been extensively used as intermediates in organic synthesis. The presence of 2 -NA in the environment is not only harmful for aquatic life but also mutagenic for human beings. In this study, we constructed transgenic rice expressing an Old Yellow Enzyme gene, ScOYE3 , from Saccharomyces cerevisiae. The ScOYE3 transgenic plants were comprehensively investigated for their biochemical responses to 2 -NA treatment and their 2 -NA phytoremediation capabilities. Our results showed that the rice seedlings exposed to 2 -NA stress, showed growth inhibition and biomass reduction. However, the transgenic plants exhibited strong tolerance to 2 -NA stress compared to wild -type plants. Ectopic expression of ScOYE3 could effectively protect transgenic plants against 2 -NA damage, which resulted in less reactive oxygen species accumulation in transgenic plants than that in wild -type plants. Our phytoremediation assay revealed that transgenic plants could eliminate more 2 -NA from the medium than wild -type plants. Moreover, omics analysis was performed in order to get a deeper insight into the mechanism of ScOYE3-mediated 2 -NA transformation in rice. Altogether, the function of ScOYE3 during 2 -NA detoxification was characterized for the first time, which serves as strong theoretical support for the phytoremediation potential of 2 -NA by Old Yellow Enzyme genes.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台