Ectopic expression of an Old Yellow Enzyme (OYE3) gene from Saccharomyces cerevisiae increases the tolerance and phytoremediation of 2-nitroaniline in rice
文献类型： 外文期刊
作者： Li, Zhenjun 1 ; Gao, Jianjie 1 ; Wang, Bo 1 ; Zhang, Hao 1 ; Tian, Yongsheng 1 ; Peng, Rihe 1 ; Yao, Quanhong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China
关键词： 2-nitroaniline; Old Yellow Enzyme; Phytoremediation; Reactive oxygen species; Saccharomyces cerevisiae
期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）
ISSN： 0378-1119
年卷期： 2024 年 906 卷
页码：
收录情况： SCI
摘要： 2-nitroaniline (2 -NA) is an environmental pollutant and has been extensively used as intermediates in organic synthesis. The presence of 2 -NA in the environment is not only harmful for aquatic life but also mutagenic for human beings. In this study, we constructed transgenic rice expressing an Old Yellow Enzyme gene, ScOYE3 , from Saccharomyces cerevisiae. The ScOYE3 transgenic plants were comprehensively investigated for their biochemical responses to 2 -NA treatment and their 2 -NA phytoremediation capabilities. Our results showed that the rice seedlings exposed to 2 -NA stress, showed growth inhibition and biomass reduction. However, the transgenic plants exhibited strong tolerance to 2 -NA stress compared to wild -type plants. Ectopic expression of ScOYE3 could effectively protect transgenic plants against 2 -NA damage, which resulted in less reactive oxygen species accumulation in transgenic plants than that in wild -type plants. Our phytoremediation assay revealed that transgenic plants could eliminate more 2 -NA from the medium than wild -type plants. Moreover, omics analysis was performed in order to get a deeper insight into the mechanism of ScOYE3-mediated 2 -NA transformation in rice. Altogether, the function of ScOYE3 during 2 -NA detoxification was characterized for the first time, which serves as strong theoretical support for the phytoremediation potential of 2 -NA by Old Yellow Enzyme genes.
- 相关文献
作者其他论文 更多>>
-
A parasitic wasp-releasing engineering to promote ecosystem services in paddy systems
作者：Zhang, Hao;You, Chun-Mei;Wang, Jin-Yan;Chen, Yi-Juan;Ji, Xiang-Yun;Wan, Nian-Feng;Woodcock, Ben A.
关键词：Ecological engineering; Biocontrol services; Chilo suppressalis; Cnaphalocrocis medinalis; Trichogramma japonicum; Wasp releasing
-
Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot
作者：Wang, Bo;Wang, Yahui;Deng, Yuanjie;Xiong, Aisheng;Wang, Bo;Yao, Quanhong
关键词：Daucus carota L.; Betanin; Biosynthesis; Metabolic engineering; Bioforti fication
-
The complete degradation of 1,2-dichloroethane in Escherichia coli by metabolic engineering
作者：Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong;Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
关键词：Bioremediation; Engineered bacteria; Environmental pollution; Metabolic engineering; Synthetic biology
-
Biosynthesis of melatonin from
l -tryptophan by an engineered microbial cell factory作者：Wang, Lijuan;Deng, Yongdong;Gao, Jianjie;Wang, Bo;Han, Hongjuan;Li, Zhenjun;Zhang, Wenhui;Wang, Yu;Fu, Xiaoyan;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing;Wang, Lijuan;Deng, Yongdong;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing
关键词：Biosynthesis; Melatonin;
l -Tryptophan (l -Trp); Escherichia coli; Metabolic engineering -
Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation
作者：Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Chen, Zhi-Feng
关键词：4-Dinitrotoluene; Re-synthesize gene; Complete degradation; Bioremediation; Escherichia coli
-
Optimizing Nitrogen Regime Improves Dry Matter and Nitrogen Accumulation during Grain Filling to Increase Rice Yield
作者：Zhou, Shenqi;Liu, Kun;Zhuo, Xinxin;Wang, Weilu;Zhang, Weiyang;Zhang, Hao;Gu, Junfei;Yang, Jianchang;Liu, Lijun;Liu, Kun
关键词：rice (Oryza sativa L.); N application rates; grain yield; dry matter accumulation; N uptake
-
Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants
作者：Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Zhu, Bo
关键词：2,4-dinitrotoluene (2,4-DNT); carbon neutrality; complete degradation; rapid phytoremediation; sustainable development goals
