您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of SNP panel for genetic diversity assessment, fingerprinting identification and backcross breeding in Brassica oleracea

文献类型： 外文期刊

作者： Yao, Xueqin 1 ; Hong, Feng 2 ; Li, Guangqing 1 ; Huang, Lei 1 ; Liu, Chunqing 1 ; Gong, Jing 1 ; Wang, Bo 2 ; Li, Juanjuan 2 ; Liu, Kede 3 ; Xie, Zhujie 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Hort, Shanghai 201403, Peoples R China

2.Wuhan GenoSeq Technol Ltd, Wuhan 430065, Peoples R China

3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

关键词： Broccoli; Single nucleotide polymorphism; GT-seq; Diversity assessment; Background recovery rate; Seed purity

期刊名称：MOLECULAR BREEDING （ 影响因子：3.0； 五年影响因子：3.0 ）

ISSN： 1380-3743

年卷期： 2025 年 45 卷 8 期

页码：

收录情况： SCI

摘要： Broccoli (Brassica oleracea L. var. italica) is a globally important vegetable due to its rich nutrients as well as its anti-cancer effect. China is the world's largest producer and exporter of broccoli. However, since the research on commercial breeding of broccoli in China started relatively late, the level of genetic breeding in our country lags behind with more than 80% seeds imported. To assist broccoli breeding with molecular markers, we re-sequenced 41 representative broccoli inbred lines at high coverage depth and identified a total of 1,348,968 SNPs. From these SNPs, a genotyping-in-thousand by sequencing (GT-seq) SNP panel composed of 700 evenly distributed high-quality SNPs was developed. We assessed the genetic diversity, population structure, and kinship of 114 B. oleracea varieties bred in different institutions including broccolis, cabbages, cauliflowers and kales with this SNP panel, and found that the genetic diversity of these varieties was somewhat limited, with an average heterozygosity of 18.35% and an average Polymorphic Information Content (PIC) of 0.26. Population structure analysis divided the varieties into two main groups, consistent with the origin from two independent domestication events. The SNP panel was also employed to screen individuals with high background recovery rates in backcross breeding. Furthermore, the SNP panel was used to test seed purity of parental inbred lines and F1 hybrids, which could expedite the entry of hybrid seeds into the market. Overall, the developed GT-seq SNP panel is a valuable tool for various aspects of B. oleracea breeding and genetics studies.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台