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Complete biodegradation of the oldest organic herbicide 2,4-Dichlorophe- noxyacetic acid by engineering Escherichia coli

文献类型： 外文期刊

作者： Wang, Yu 1 ; Tian, Yong-Sheng 1 ; Gao, Jian-Jie 1 ; Xu, Jing 1 ; Li, Zhen-Jun 1 ; Fu, Xiao-Yan 1 ; Han, Hong-Juan 1 ; Wang, Li-Juan 1 ; Zhang, Wen-Hui 1 ; Deng, Yong-Dong 1 ; Qian, Cen 1 ; Zuo, Zhi-Hao 1 ; Wang, Bo 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China

关键词： 24-Dichlorophenoxyacetic acid; Biodegradation; Environmental pollution; Engineered bacteria; Synthetic biology

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2023 年 451 卷

页码：

收录情况： SCI

摘要： After nearly 80 years of extensive application, the oldest organic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has caused many problems of environmental pollution and ecological deterioration. Bioremediation is an ideal method for pollutant treatment. However, difficult screening and preparation of efficient degradation bacteria have largely hindered its application in 2,4-D remediation. We have created a novel engineering Escherichia coli with a reconstructed complete degradation pathway of 2,4-D to solve the problem of screening highly efficient degradation bacteria in this study. The results of fluorescence quantitative PCR demonstrated that all nine genes in the degradation pathway were successfully expressed in the engineered strain. The engineered strains can quickly and completely degrade 0.5 mM 2, 4-D within 6 h. Inspiring, the engineered strains grew with 2,4-D as the sole carbon source. By using the isotope tracing method, the metabolites of 2,4-D were found incorporated into the tricarboxylic acid cycle in the engineering strain. Scanning electron microscopy showed that 2,4-D had less damage on the engineered bacteria than the wild-type strain. Engineered strain can also rapidly and completely remedy 2,4-D pollution in natural water and soil. Assembling the metabolic pathways of pollutants through synthetic biology was an effective method to create pollutant-degrading bacteria for bioremediation.

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