文献类型： 外文期刊
作者： Chen, Liqing 1 ; Wang, Yu 1 ; Fan, Lixia 1 ; Yang, Nuo 1 ; Zeng, Jifeng 1 ; Guo, Guiying 1 ; Li, Qian 1 ; Wang, Peng 4 ; Zeng, Weixin 5 ; Zheng, Jiping 1 ;
作者机构： 1.Hainan Univ, Sch Life Sci, Lab Microbial Engn Infect & Immun, Haikou 570228, Peoples R China
2.Hainan Univ, One Hlth Inst, Haikou 570228, Peoples R China
3.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Biotechnol Res Inst, Shanghai 201106, Peoples R China
4.Acad Mil Med Sci, Inst Biotechnol, Beijing 100071, Peoples R China
5.Beijing Shijitan Hosp, Dept Pharm, Beijing 100038, Peoples R China
关键词： Aeromonas dhakensis; KdpE; Attenuated virulence; Histopathology; Zebrafish
期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）
ISSN： 0044-8486
年卷期： 2023 年 568 卷
页码：
收录情况： SCI
摘要： Aeromonas dhakensis is an emerging pathogen that is harmful to humans and aquaculture in tropical areas. The aim of this study was to understand the role of the two-component signal transduction system KdpDE in regu-lating the virulence of this species. A Delta kdpE mutant was generated via a markerless knockout. Phenotype analysis showed that the mutant was attenuated in growth, biofilm formation, mobility, ECPase activity, and resistance to H2O2 and antimicrobial peptides under conditions of potassium depletion. A challenge experiment to analyze the virulence of A. dhakensis in zebrafish, via intraperitoneal injection, demonstrated that the clinicopathological signs of infection were dropsy, tail and/or fin bleeding, hepatonecrosis, spleen congestion, and nephrohemia. The LD50 value of the mutant (9.02 x 106 CFU/ml) was 15.6-fold higher than that of the wild-type strain (5.77 x 105 CFU/ml). The survival rate of zebrafish was higher after double immunization (76.7%) than after single im-munization with the mutant (36.7%). These data reveal for the first time that KdpDE is associated with the virulence of A. dhakensis. The Delta kdpE mutant is attenuated in virulence and shows high protective efficacy in adult zebrafish after double immunization, and is therefore a promising attenuated vaccine candidate.
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