您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Flower-specific expression of Arabidopsis PCS1 driven by AGAMOUS second intron in tobacco decreases the fertility of transgenic plants

文献类型： 外文期刊

作者： Xu, Xiangbin 1 ; Bian, Jufang 1 ; Liu, Songbai 1 ; Song, Hongmiao 2 ; Shi, Nongnong 1 ; Tao, Yuezhi 2 ; Wang, Huizhong 1 ;

作者机构： 1.Hangzhou Normal Univ, Key Lab Biochem & Mol Biol, Hangzhou 310018, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Peoples R China

关键词： Arabidopsis thaliana;tobacco.;AG second intron;PCS1;Sterility;Internet resource.

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The PROMOTION OF CELL SURVIVAL 1 (PCS1) gene, encoding an aspartic protease, has an important role in determining the fate of cells in embryonic development and reproduction processes in Arabidopsis. To explore the potential function of the PCS1 gene in generating reproductive sterility, we placed the PCS1 gene under the control of an 1,869-bp nucleotide sequence from the 3o end of the second intron (AG-I) of Arabidopsis AGAMOUS and CaMV 35S (-60) minimal promoter [AG-I-35S (-60)::PCS1], and introduced it into tobacco. RT-PCR results demonstrated that the PCS1 gene driven by AG-I-35S (-60) chimeric promoter was expressed only in anthers and carpels in the reproductive tissues of transgenic tobacco. Compared to wild-type plants, all AG-I-35S (-60) and AG-I-35S (-60)::PCS1 transgenic lines showed a normal phenotype throughout the vegetative growth phase. However, during the reproductive stage, most AG-I-35S (-60)::PCS1 transgenic plant anthers displayed delayed dehiscence, failed dehiscence, petalody and hypoplasia, and the pollen grains had different shapes and sizes with a distorted, shrunken, or collapsed morphology. Moreover, three transgenic lines, PCS1-1, PCS1-3 and PCS1-4, showed higher sterility than wild-type and AG-I-35S (-60) transgenic plants, respectively. These results showed that the construct of AG-I-35S (-60)::PCS1 was partially effective at preventing seed set and provided a novel sterility strategy.

  • 相关文献

[1]Silicon-enhanced resistance to rice blast is attributed to silicon-mediated defence resistance and its role as physical barrier. Sun, Wanchun,Zhang, Jie,Fan, Qionghua,Xue, Gaofeng,Li, Zhaojun,Liang, Yongchao,Sun, Wanchun.

[2]A 796 bp PsPR10 gene promoter fragment increased root-specific expression of the GUS reporter gene under the abiotic stresses and signal molecules in tobacco. Xu, Xiangbin,Guo, Sai,Chen, Kai,Liu, Junjun,Wang, Huizhong,Song, Hongmiao,Liu, Junjun,Guo, Longbiao,Qian, Qian.

[3]Molecular analysis shows differential expression of R-spondin1 in zebrafish (Danio rerio) gonads. Zhang, Yanmei,Li, Fei,Liu, Jiangdong,Liu, Na,Yu, Qixing,Sun, Dongchang.

[4]Molecular characterization and association analysis of porcine adipose triglyceride lipase (PNPLA2) gene. Dai, Li He,Xiong, Yuan Zhu,Jiang, Si Wen,Dai, Li He,Chen, Jun Feng.

[5]Identification of an aminopeptidase from the skeletal muscle of grass carp (Ctenopharyngodon idellus). Liu, Bing-Xin,Sun, Le-Chang,Su, Wen-Jin,Cao, Min-Jie,Zhou, Li-Gen,Hara, Kenji.

[6]Development of wild barley-derived DArT markers and their integration into a barley consensus map. Alsop, B. P.,Steffenson, B. J.,Wenzl, P.,Kilian, A.,Farre, A.,Romagosa, I.,Wang, J. M.,Zhou, M. X..

[7]Abiotic Stresses and Phytohormones Regulate Expression of FAD2 Gene in Arabidopsis thaliana. Yuan Si-wei,Wu Xue-long,Liu Zhi-hong,Huang Rui-zhi,Yuan Si-wei,Luo Hong-bing. 2012

[8]NRAMP2, a trans-Golgi network-localized manganese transporter, is required for Arabidopsis root growth under manganese deficiency. Gao, Huiling,Xie, Wenxiang,Yang, Changhong,Xu, Jingyi,Huang, Chao-Feng,Gao, Huiling,Xie, Wenxiang,Yang, Changhong,Xu, Jingyi,Huang, Chao-Feng,Gao, Huiling,Xie, Wenxiang,Yang, Changhong,Xu, Jingyi,Huang, Chao-Feng,Li, Jingjun,Chen, Xi,Wang, Hua. 2018

[9]The Function of LPR1 is Controlled by an Element in the Promoter and is Independent of SUMO E3 Ligase SIZ1 in Response to Low Pi Stress in Arabidopsis thaliana. Wang, Xuemin,Du, Guankui,Wang, Xuming,Meng, Yijun,Li, Yiyi,Wu, Ping,Yi, Keke,Wang, Xuemin,Du, Guankui,Meng, Yijun,Wu, Ping,Yi, Keke,Du, Guankui,Meng, Yijun,Wu, Ping,Yi, Keke,Wang, Xuming. 2010

[10]Expression of five AtHsp90 genes in Saccharomyces cerevisiae reveals functional differences of AtHsp90s under abiotic stresses. Song, Hongmiao,Fan, Pengxiang,Shi, Wuliang,Li, Yinxin,Song, Hongmiao,Zhao, Rongmin. 2010

[11]Pretreatment with low-dose gamma irradiation enhances tolerance to the stress of cadmium and lead in Arabidopsis thaliana seedlings. Qi, Wencai,Wang, Lin,Xu, Hangbo,Jiao, Zhen,Zhang, Liang,Jin, Qingsheng.

[12]GLABROUS INFLORESCENCE STEMS3 (GIS3) regulates trichome initiation and development in Arabidopsis. Sun, Lili,Zhang, Aidong,Zhao, Yongqin,Yan, An,Bao, Shengjie,Gan, Yinbo,Zhou, Zhongjing,Yu, Hao,Yu, Hao.

[13]Functional characterization of AtHsp90.3 in Saccharomyces cerevisiae and Arabidopsis thaliana under heat stress. Xu, Xiangbin,Song, Hongmiao,Zhou, Zhenhua,Shi, Nongnong,Ying, Qicai,Wang, Huizhong,Song, Hongmiao.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台