文献类型： 外文期刊
作者： Hao, Juan 1 ; Jiao, Kaili 1 ; Yu, Chenliang 3 ; Guo, Hong 1 ; Zhu, Yujia 1 ; Yang, Xiao 1 ; Zhang, Siyang 1 ; Zhang, Lei; 1 ;
作者机构： 1.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China
2.Hangzhou Normal Univ, Zhejiang Prov Key Lab Genet Improvement & Qual Co, Hangzhou 310036, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Vegetable Res Inst, Hangzhou 310021, Zhejiang, Peoples R China
4.Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA
5.Hangzhou Normal Univ, Coll Life & Environm Sci, Key Lab Hangzhou City Ecosyst Protect & Restorat, Hangzhou 310036, Zhejiang, Peoples R China
关键词： Taxus media; Species specific; SCoT marker; SCAR
期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）
ISSN： 0006-2928
年卷期： 2018 年 56 卷 3 期
页码：
收录情况： SCI
摘要： Taxus media is an important species in the family Taxaceae with high medicinal and commercial value. Overexploitation and illegal trade have led T. media to a severe threat of extinction. In addition, T. media and other Taxus species have similar morphological traits and are easily misidentified, particularly during the seedling stage. The purpose of this study is to develop a species-specific marker for T. media. Through a screening of 36 start codon targeted (SCoT) polymorphism primers, among 15 individuals of 4 Taxus species (T. media, T. chinensis, T. cuspidate and T. fuana), a clear species-specific DNA fragment (amplified by primer SCoT3) for T. media was identified. After isolation and sequencing, a DNA sequence with 530 bp was obtained. Based on this DNA fragment, a primer pair for the sequence-characterized amplified region marker was designed and named MHSF/MHSR. PCR analysis with primer pair MHSF/MHSR revealed a clear amplified band for all individuals of T. media but not for T. chinensis, T. cuspidate and T. fuana. Therefore, this marker can be used as a quick, efficient and reliable tool to identify T. media among other related Taxus species. The results of this study will lay an important foundation for the protection and management of T. media as a natural resource.
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