您好,欢迎访问中国水产科学研究院 机构知识库!

Molecular cloning, characterization and expression analysis of thrombospondin gene from Penaeus monodon

文献类型: 外文期刊

作者: Zhou, FaLin 1 ; Zheng, Liming 1 ; Zhang, Dianchang 1 ; Huang, JianHua 1 ; Qiu, Lihua 1 ; Yang, QiBin 1 ; Jiang, ShiGui 1 ;

作者机构: 1.CAFS, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.S China Agr Univ, Inst Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

关键词: Molecular cloning;Thrombospondin;mRNA expression;Penaeus monodon

期刊名称:MARINE GENOMICS ( 影响因子:1.71; 五年影响因子:1.969 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In present study, a thrombospondin gene was obtained from the ovary and neurosecretory organ in eyestalk cDNA library of black tiger prawn (Penaeus monodon). The full-length P. monodon thrombospondin (PmTSP) cDNA contained a 5 untranslated region (UTR) of 9 bp, an open reading frame (ORF) of 2778 bp encoding a polypeptide of 925 amino acids with molecular mass 100.57 kDa, and a 3UTR of 99 bp. ScanProsite analysis indicated that PmTSP contained four chitin-binding type-II domains, an EGF-like domain, eight thrombospondin type-III repeats and one thrombospondin C-terminal domain. Homology analysis of the deduced amino acid sequence of the PmTSP with other known TSP sequences by MatGAT software revealed that the PmTSP shows very high homology with the sequences of Fennerpenaeus chinensis (89.9% similarity, 83.8% identity). Analysis of the tissue expression pattern of the PmTSP gene showed that the PmTSP mRNA was expressed in all tested tissues, including hepatopancreas, ovary, muscle, intestine, neurosecretory organ in eyestalk, neurosecretory organ in brain, stomach, and heart, with highest level in the ovary. Furthermore, the PmTSP expression was found to be of high level in six development stages of the ovary. The results indicated that PmTSP might play an important role in ovarian development.

  • 相关文献

[1]Molecular Analysis of a Selenoprotein M Gene from Penaeus monodon and its Expression at Different Ovarian Stages of Development. Zhou, FaLin,Qiu, Lihua,Huang, JianHua,Yang, QiBin,Fu, Mingjun,Jiang, ShiGui,Zhou, FaLin,Qiu, Lihua,Huang, JianHua,Yang, QiBin,Fu, Mingjun,Jiang, ShiGui. 2015

[2]Molecular analysis of a ras-like nuclear (Ran) gene from Penaeus monodon and its expression at the different ovarian stages of development. Zhou, FaLin,Zheng, Liming,Yang, QiBin,Qiu, Lihua,Huang, JianHua,Su, Tiannfeng,Jiang, ShiGui,Zhou, FaLin,Zheng, Liming.

[3]Molecular cloning and mRNA expression of cathepsin C gene in black tiger shrimp (Penaeus monodon). Jiang, Shigui.

[4]The response of glutathione peroxidase 1 and glutathione peroxidase 7 under different oxidative stresses in black tiger shrimp, Penaeus monodon. Liu, Wei,Zhao, Chao,Wang, Pengfei,Qiu, Lihua,Liu, Wei,Lin, Heizhao,Qiu, Lihua,Wang, Shu. 2018

[5]Gene characteristics, immune and stress responses of PmPrx1 in black tiger shrimp (Penaeus monodon): Insights from exposure to pathogenic bacteria and toxic environmental stressors. Bu, Ruiqian,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Bu, Ruiqian,Bu, Ruiqian,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua,Bao, Weiyang.

[6]The anti-stresses capability of GRP78 in Penaeus monodon: Evidence from in vitro and in vivo studies. Li, Lili,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Li, Lili,Li, Lili,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua. 2018

[7]Molecular analysis of the QM gene from Penaeus monodon and its expression on the different ovarian stages of development. Zhou, FaLin,Jiang, ShiGui,Huang, JianHua,Qiu, Lihua,Zhang, Dianchang,Su, Tiannfeng,Zhou, FaLin.

[8]Cloning, characterization, expression analysis and RNAi of Retinoblastoma-like gene from black tiger shrimp (Penaeus monodon). Xie, Bobo,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Xie, Bobo,Xie, Bobo,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua.

[9]南海西北部水域斑节对虾资源的调查研究. 钟振如,李辉权,张月平,陈敏. 1999

[10]Molecular cloning and expression of grass carp MyoD in yeast Pichia pastoris. Wang, Lixin,Bai, Junjie,Luo, Jianren,Chen, Hong,Ye, Xing,Jian, Qing,Lao, Haihua. 2007

[11]The characterization, expression and activity analysis of superoxide dismutases (SODs) from Procambarus clarkii. Meng, Qingguo,Chen, Jing,Xu, Chenchao,Wang, Ya,Wang, Tingting,Zhai, Xiaoting,Gu, Wei,Wang, Wen,Meng, Qingguo,Chen, Jing,Xu, Chenchao,Wang, Ya,Wang, Tingting,Zhai, Xiaoting,Gu, Wei,Wang, Wen,Huang, Yanqing. 2013

[12]Molecular cloning and gene/protein expression of FAT/CD36 from grass carp (Ctenopharyngodon idella) and the regulation of its expression by dietary energy. Tian, Juan,Liu, Wei,Wu, Fan,Yu, Lijuan,Lu, Xing,Yang, Chang-Geng,Jiang, Ming,Wen, Hua,Tian, Juan,Jiang, Ming,Gao, Weihua.

[13]Molecular Cloning of Cathelicidin-like cDNA from Andrias davidianus. Yan, M.,Tian, H. F.,Hu, Q. M.,Xiao, H. B.. 2018

[14]Molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells. Gao, Quanxin,Zhang, Chenjie,Min, Minghua,Peng, Shiming,Shi, Zhaohong,Xiao, Yingping.

[15]Molecular cloning, tissue and embryonic development expression, and appetite regulatory effect of pancreatic peptide Y in Coilia nasus. Yang, Siyu,Xu, Gangchun,Xu, Pao,Xu, Gangchun,Du, Fukuan,Xu, Pao.

[16]Molecular cloning and characterization of the lipopolysaccharide and beta-1,3-glucan binding protein from red claw crayfish, Cherax quadricarinatus. Hou, Libo,Liu, Xiaoqian,Jing, Yunting,Zhang, Jia,Gu, Wei,Meng, Qingguo,Wang, Wen,Hou, Libo,Liu, Xiaoqian,Jing, Yunting,Zhang, Jia,Gu, Wei,Meng, Qingguo,Wang, Wen,Ding, Zhengfeng,Huang, Yanqing.

[17]Molecular cloning and sequence analysis of methionine adenosyltransferase from the economic seaweed Undaria pinnatifida. Qiao, Kun,Li, Hong Yan,Tong, Shao Ming,Hou, He Sheng,Li, Shi Guo.

[18]Comparison of effect of chitin, chitosan, chitosan oligosaccharide and N-acetyl-D-glucosamine on growth performance, antioxidant defenses and oxidative stress status of Penaeus monodon. Niu, Jin,Lin, Hei-Zhao,Jiang, Shi-Gui,Chen, Xu,Wu, Kai-Chang,Liu, Yong-Jian,Tian, Li-Xia,Wang, Sheng. 2013

[19]Cloning and characterization of PHGPx and its synergistic role with p53 in mediating stress in Penaeus monodon. Li, Fuxiang,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Li, Fuxiang,Li, Fuxiang,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua,Bao, Weiyang. 2017

[20]Dietary values of astaxanthin and canthaxanthin in Penaeus monodon in the presence and absence of cholesterol supplementation: effect on growth, nutrient digestibility and tissue carotenoid composition. Niu, Jin,Li, Chun-Hou,Chen, Xu,Huang, Zhong,Lin, Hei-Zhao,Liu, Yong-Jian,Tian, Li-Xia. 2012

作者其他论文 更多>>