Molecular cloning, characterization and expression analysis of thrombospondin gene from Penaeus monodon
文献类型: 外文期刊
作者: Zhou, FaLin 1 ; Zheng, Liming 1 ; Zhang, Dianchang 1 ; Huang, JianHua 1 ; Qiu, Lihua 1 ; Yang, QiBin 1 ; Jiang, ShiGui 1 ;
作者机构: 1.CAFS, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.S China Agr Univ, Inst Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
关键词: Molecular cloning;Thrombospondin;mRNA expression;Penaeus monodon
期刊名称:MARINE GENOMICS ( 影响因子:1.71; 五年影响因子:1.969 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In present study, a thrombospondin gene was obtained from the ovary and neurosecretory organ in eyestalk cDNA library of black tiger prawn (Penaeus monodon). The full-length P. monodon thrombospondin (PmTSP) cDNA contained a 5 untranslated region (UTR) of 9 bp, an open reading frame (ORF) of 2778 bp encoding a polypeptide of 925 amino acids with molecular mass 100.57 kDa, and a 3UTR of 99 bp. ScanProsite analysis indicated that PmTSP contained four chitin-binding type-II domains, an EGF-like domain, eight thrombospondin type-III repeats and one thrombospondin C-terminal domain. Homology analysis of the deduced amino acid sequence of the PmTSP with other known TSP sequences by MatGAT software revealed that the PmTSP shows very high homology with the sequences of Fennerpenaeus chinensis (89.9% similarity, 83.8% identity). Analysis of the tissue expression pattern of the PmTSP gene showed that the PmTSP mRNA was expressed in all tested tissues, including hepatopancreas, ovary, muscle, intestine, neurosecretory organ in eyestalk, neurosecretory organ in brain, stomach, and heart, with highest level in the ovary. Furthermore, the PmTSP expression was found to be of high level in six development stages of the ovary. The results indicated that PmTSP might play an important role in ovarian development.
- 相关文献
作者其他论文 更多>>
-
Comparative analysis of the structural and compositional change of spotted sea bass (Lateolabrax maculatus) gut microflora following Aeromonas veronii infection and the effects of Lactobacillus plantarum on these changes
作者:Lin, Changhong;Lin, Changhong;Lin, Changhong;Zhang, Bo;Zhang, Boa;Qiu, Lihua;Zhang, Bo;Wang, Pengfei;Zhang, Boa;Yan, Lulu;Zhao, Chao;Qiu, Lihua;Zhang, Bo;Wang, Pengfei;Zhang, Boa;Yan, Lulu;Zhao, Chao;Qiu, Lihua;Zhang, Bo;Wang, Pengfei;Zhang, Boa;Zhao, Chao;Zhao, Chao
关键词:Lateolabrax maculatus; Aeromonas veronii; Gut microbiota; intestinal flora; pathogen.
-
Effects of Partial Substitution of Fish Meal with Soybean Products and Chicken Meal on Growth, Antioxidant Capacity and Intestinal Microbiota of Penaeus monodon
作者:Yang, Wanli;Jiang, Song;Yang, Qibin;Huang, Jianhua;Yang, Yukai;Jiang, Song;Yang, Qibin;Shi, Jianzhi;Li, Yundong;Zhou, Falin;Jiang, Song;Yang, Qibin
关键词:Penaeus monodon; growth performance; antioxidant capacity; intestinal microbiota
-
Evaluation of Genetic Parameters and Comparison of Stress Tolerance Traits in Different Strains of Litopenaeus vannamei
作者:Shi, Miao;Jiang, Song;Yang, Qibin;Zhou, Falin;Shi, Miao;Jiang, Song;Yang, Qibin;Shi, Miao;Jiang, Song;Shi, Jianzhi;Yang, Qibin;Huang, Jianhua;Li, Yundong;Yang, Lishi
关键词:Litopenaeus vannamei; ammonia-N; pH; salinity; tolerance; heritability; genetic parameter
-
Rhodiola rosea L. improved intestinal digestive enzyme activities, inflammatory response, barrier and microbiota dysbiosis in Lateolabrax maculatus juveniles fed with high-carbohydrate diets
作者:Zheng, Luzhe;Wang, Zhanzhan;Zhang, Bo;Zhao, Chao;Qiu, Lihua;Zhou, Chuanpeng;Zheng, Luzhe;Zhao, Chao;Yan, Lulu;Wang, Pengfei;Wang, Jun;Wang, Yun;Zhou, Chuanpeng;Yan, Lulu;Wang, Pengfei;Wang, Jun;Wang, Yun;Lin, Heizhao;Zhou, Chuanpeng;Lin, Heizhao
关键词:Intestinal barrier; Intestinal immunity; Inflammatory; Intestinal microbiota; Rhodiola rosea L.; Lateolabrax maculatus
-
Effects of Three Feed Additives in Low Fish Meal Diet on Growth, Antioxidant Capacity and Intestinal Microbiota of Penaeus monodon
作者:Yang, Wanli;Jiang, Song;Yang, Qibin;Huang, Jianhua;Yang, Yukai;Jiang, Song;Yang, Qibin;Shi, Jianzhi;Li, Yundong;Zhou, Falin;Jiang, Song;Yang, Qibin
关键词:Penaeus monodon; growth performance; antioxidant capacity; intestinal microbiota
-
Effects of Dietary Gracilaria lichenoides and Bacillus amyloliquefaciens on Growth Performance, Antioxidant Capacity, and Intestinal Health of Penaeus monodon
作者:Tian, Jialin;Yan, Hailiang;Tian, Jialin;Wang, Yun;Huang, Jianhua;Yan, Hailiang;Duan, Yafei;Wang, Jun;Zhou, Chuangpeng;Wang, Yun;Wang, Jun;Zhou, Chuangpeng;Huang, Zhong
关键词:Penaeus monodon; Gracilaria lichenoides; Bacillus amyloliquefaciens; growth; antioxidant capacity; intestine microbiota; lipid metabolism
-
Identification of microRNAs in black tiger shrimp (Penaeus monodon) under acute low-salinity stress
作者:Shi, Jianzhi;Yang, Qibin;Zhou, Falin;Shi, Jianzhi;Jiang, Song;Li, Yundong;Yang, Lishi;Jiang, Shigui;Zhou, Falin;Huang, Jianhua
关键词:low-salinity; high-throughput sequencing; microRNA; stress response; Penaeus monodon